Abstract

SILVA, N.M.V. et al. Genetic polymorphism of the leptin and receptor growth hormone in goats. Arch. zootec. [online]. 2012, vol.61, n.234, pp.187-195. ISSN 1885-4494.  https://dx.doi.org/10.4321/S0004-05922012000200003.

This study aimed to evaluate the relationship between leptin gene polymorphism (LEP), specifically exon 2, and microsatellite of the growth hormone receptor (GHR-SSR) with the growth characteristics of Anglo-Nubian and Boer goats to identify useful markers for selecting animals with high genetic merit. The Toolkit program was used for obtaining the allele frequencies and heterozygosity. Hardy-Weinberg equilibrium test was performed with GENEPOP software. Markers were in desequilibrium in all populations studied. Observed heterozygosity values for LEP were greater than expected heterozygosity. All animals showed the same electrophoretic pattern with two alleles (150 and 152 bp). On GHR locus was detected five alleles ranging from 90 to 125 bp. Birth weight (BW) and weaning weight (PD) were used to evaluate the influence of polymorphic fragments on the animal growth. They were analyzed by GLM procedure of SAS software. The genotypes showed a significant effect on birth (BW) and weaning weight (PD). It is necessary to increase the number of samples to confirm the relationship among GHR and leptina polymorphic fragments GHR and growth characteristics.

Keywords : Molecular markers.

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