Abstract

COSME, Ángel et al. Diagnosis of Whipple's disease using molecular biology techniques. Rev. esp. enferm. dig. [online]. 2011, vol.103, n.4, pp.213-217. ISSN 1130-0108.  https://dx.doi.org/10.4321/S1130-01082011000400009.

extraintestinal manifestations, species-specific PCR using different sequences of the T. whippleii genome from different tissue types and biological fluids is recommended. The diagnosis of Whipple's disease (WD) is based on the existence of clinical signs and symptoms compatible with the disease and in the presence of PAS-positive diastase-resistant granules in the macrophages of the small intestine. If there is suspicion of the disease but no histological findings or only isolated This study reports two cases: the first patient had diarrhea and the disease was suspected after an endoscopic examination of the ileum, while the second patient had multi-systemic manifestations, particularly abdominal, thoracic, and peripheral lymphadenopathies. In both cases, the diagnosis was confirmed using molecular biology techniques to samples from the small intestine or from a retroperineal lymph node, respectively.

Keywords : Whipple's disease; Molecular diagnosis; Polymerase chain reaction; Tropheryma whippleii; Enfermedad de Whipple; Diagnóstico molecular; Reacción en cadena de polimerasa; Tropheryma Whippleii.

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