Abstract

OBREGON, Virginia et al.  Peculiarities of the DNA of MM1, a temperate phage of Streptococcus pneumoniae. INT. MICROBIOL. [online]. 2004, vol.7, n.2, pp.133-137. ISSN 1139-6709.

The abundant presence of temperate phages in the chromosomes of clinical isolates of Streptococcus pneumoniae has been well documented. The genome of MM1, a temperate phage of pneumococcus, has been isolated as a DNA-protein complex. The protein is covalently bound to the DNA, was iodinated in vitro with Na¹²⁵I, and has an Mr of 22,000. Electron microscopy and enzymatic analyses revealed that the MM1 genome is a linear, circularly permuted, terminally redundant collection of double-stranded DNA molecules packaged via a headful mechanism. The location of the pac site appears to be downstream of the terminase, between orf32 and orf34 of the MM1 genome.

Keywords : pneumococcus; DNA-protein complex; pac site; temperate phages.

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