Abstract

LEYVA HUERTA, E.R. et al. Lactate dehidrogenase activity in gingival crevicular fluid and saliva of smoker with chronic periodontitis. Avances en Periodoncia [online]. 2009, vol.21, n.1, pp.21-26. ISSN 2340-3209.

The response of the body to periodontal disease includes the production of intra-cellular enzymes like Lactate Dehydrogenase (LDH) which is released by damaged periodontal cells. The LDH can be found in the gingival crevicular fluid (GCF) and saliva as a result of cellular death and damage. Objective: To compare LDH activity in GCF and saliva of smoker and non-smoker patients with Chronic Periodontitis. Methods: 15 male patients (30 to 50 years of age) participated in this study; 6 smokers with Chronic Periodontitis, 6 non-smokers with Chronic Periodontitis and 3 health subjects. Samples of stimulated total saliva as well as four samples of gingival crevicular fluid while probing periodontal pockets (= 6 mm deep) with standarized #30 endodontic paper points were obtained. The enzymatic activity of LDH was recorded and was quantified by espectrophotometry. Results: GCF volume was 0.78 µL in health subjects, 1.12 µL non-smokers with Chronic Periodontitis and 1.32 µL smokers with Chronic Periodontitis. LDH activity in saliva of smoker was 1.7895 mM. non-smoker patients with Chronic Periodontitis 1.325 and health subjects 0.845 mM. In FCG the activity was 0.4568 mM of health subjects , non-smokers with Chronic Periodontitis 0.987 and smoker with Chronic Periodontitis was 1.2546 mM. The enzymatic activity of LDH in gingival crevicular fluid and saliva was greater in smoking patients compared to non-smoking patients. Conclusions: These results suggest that LDH activity in saliva and gingival crevicular fluid can be used to evaluate the progression of periodontal disease as well as the effects of smoking.

Keywords : Lactate Dehidrogenase; gingival crevicular fluid; saliva; chronic periodontitis.

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