Abstract

PEREZ RICO, A. et al. Protocol for DNA extraction in batches of 10 mosquitoes for the identification of Plasmodium spp. thorough qPCR. Sanid. Mil. [online]. 2013, vol.69, n.2, pp.78-81. ISSN 1887-8571.  https://dx.doi.org/10.4321/S1887-85712013000200003.

Troops deployed in operational areas where malaria is endemic, need accurate information on the health risk for this disease to make decisions about the most appropriate prevention measures. The carrier status of a mosquito is classically determined by the presence or absence of sporozoites of Plasmodium spp. in the salivary glands. Protocols based on DNA amplification in real time (qPCR) are very sensitive, however there are difficulties in qPCR due to inhibitors present in tissues of the mosquito, therefore it is necessary to work one by one. In this paper we design a qPCR to amplify a preserved region among different species of mosquitoes and other Diptera, in order to compare various DNA extraction protocols to determine the most efficient one when processing batches of 10 mosquitoes.

Keywords : Malaria; Diptera; Anopheles; Real-Time Polymerase Chain Reaction; Ribosomal DNA.

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