My SciELO
Services on Demand
Journal
Article
Indicators
- Cited by SciELO
- Access statistics
Related links
- Cited by Google
- Similars in SciELO
- Similars in Google
Share
Sanidad Militar
Print version ISSN 1887-8571
Abstract
CARBAJO-OTERO, A et al. Biofilms in military food facilities. Comparison of rapid surveillance and verification techniques. Sanid. Mil. [online]. 2022, vol.78, n.2, pp.89-94. Epub Jan 20, 2023. ISSN 1887-8571. https://dx.doi.org/10.4321/s1887-85712022000200006.
Introduction:
Biofilm-forming pathogens increase the risk of biological food contamination in food industries, thus it is necessary to quantify and qualify the dangers present in them.
Objective:
To demonstrate the presence of bacterial biofilms on the work surfaces of military food facilities using different rapid hygienic control techniques.
Materials and methods:
An amount of 550 samples were analyzed among 10 military food facilities: Each one had 5 sampling points (n = 50) and in each of them 11 samples were obtainded on a surface of 100 cm2 to assess the presence of biofilms and to establish the correlation between different analytical techniques: determination of adenosin triphosphate (ATP) using 3M Clean-Trace ™ NG3 luminometer, protein residue titration with Clean-Trace ™ Surface Protein Plus swabs, mesophilic aerobic microorganism (AC) counts using TEMPO® and Petrifilm ™ system, and visualization of biofilms using enzymatic reaction with Biofinder®.
Results:
A statistically significant correlation (p <0.05) was found between ATP determinations and the presence of biofilms and protein residues. Linearity with statistical significance (R2 0.422, p <0.05) was also found between ATP and AC count by TEMPO®. Correlation and concordance between AC counts were observed using the TEMPO® system and through Petrifilm ™ (intraclass correlation coefficient 0.463, 95% CI: 0.179-0.673, p <0.05). At no sampling point (SP) were pathogenic bacteria detected.
Conclusion:
Combining quick techniques should be considered in surveillance procedures of Hazard Analysis and Critical Control Points (HACCP).
Keywords : biofilm; cross contamination; ATP; cleaning and disinfection; HACCP.