SciELO - Scientific Electronic Library Online

 
vol.6 número7Evaluación del crecimiento radial de Lactarius volemus en medio semisólidoAbordaje no farmacológico del dolor en pediatría desde la perspectiva de enfermería: aplicación de materiales audiovisuales y buzzy® índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Compartir


Journal of Negative and No Positive Results

versión On-line ISSN 2529-850X

Resumen

MATIAS-LUIS, Gabriel et al. Quantification of specific lectin activity in teosinte Zea diploperennis. JONNPR [online]. 2021, vol.6, n.7, pp.941-950.  Epub 18-Dic-2023. ISSN 2529-850X.  https://dx.doi.org/10.19230/jonnpr.3860.

Objective.

To evaluate the specific activity of lectin in healthy and infected teosinte Zea diploperennis with Ustilago maydis.

Materials and methods.

Zea diploperennis seedlings of 6 days of growth were inoculated with Ustilago maydis. Total protein concentration and hemagglutinating activity of crude extracts of healthy and infected teosinte were evaluated in microtiter plates with 3% type O red cells following the technique of serial double dilutions for 6 days.

Results.

The concentration of total proteins increases in healthy coleoptile during each day of its growth. Not so in infected teosinte where the curve shows a downward trend from the moment of inoculation. The specific lectin activity decreases in both cases from the first day of quantification.

Conclusions.

The evident reduction in lectin activity in infected teosinte compared to healthy teosinte could explain the susceptibility of this teosinte to said phytopathogen. Although the participation of the teosinte and corn lectins in the defense mechanism against Ustilago maydis has not yet been clarified, the results obtained contribute to the understanding of the effect that the concentration of lectin and protein can have on resistance in teosinte.

Palabras clave : Lectin activity; total protein; Zea diploperennis; Ustilago maydis.

        · resumen en Español     · texto en Español     · Español ( pdf )