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Actas Urológicas Españolas
versão impressa ISSN 0210-4806
Resumo
LABORDA RODRIGUEZ, A. et al. Histopathological validation of tissue-microarray technology in urotelial cancer: Our experience. Actas Urol Esp [online]. 2004, vol.28, n.3, pp.215-220. ISSN 0210-4806.
INTRODUCTION: The array technology offers: a big advance to clinic and basic investigator, it provides a variety of technics (immunohystochemistry, FISH, proteomics) to undestand the molecular mechanisms of cancer. It offers scale economy in reagents versus the conventional methods. Array most be ratified because the sample is so reduced. MATERIAL AND METHODS: 52 consecutive cases have been cloosen from paraffin blocks of bladder and ureteral cancer which are 5-7 years old, a tissue array has been made; disks have been arranged in lines and columns, in an aleatory way, in order to guide its reading. It has been evaluated by a pathologist with any relation to specimen selection. RESULTS: 87 sheets ha been obtained. Number 1 has been dyed with HE. Has been discrepancy in 27% of samples stage. Has not been a discrepancy in hystopathologic diagnostic. There is no samples representation in 11 points (17%). DISCUSSION: Our results offer good results in samples validation. The samples antigenicity of tissue is conserved. Array samples represent a 97%, similary to all unit of conventional sections of the specimen.
Palavras-chave : Microarray; Bladder cancer; Renal pelvis cancer; FISH; Immunohistochemistry.