Resumo

VILLEGAS, MR.; ACEVEDO, ML.; MIRANDA, J.  e  PINTO, EA.. Validation of techniques for detection of blood, human blood and sanguineous group ABO under different supports and conditions with forensic purposes. Cuad. med. forense [online]. 2005, n.42, pp.267-274. ISSN 1988-611X.

Blood stains found at the scene of the crime have become an essential element when solving judicial investigations. To establish its origin and sanguineous group contributes to useful information in the inclusion process or exclusion of suspects or victims. In our study, we examined the Pyramidone, Luminol, and Phenolphthalein presumptive tests in order to establish their sensitivity (with different degrees of interference) and in different support, temperature, time, and storage conditions. The same was carried out to evaluate the agglutination inhibition, absorption-elution and Takayama tests, also used to establish blood origin and type group. In general terms, both the presumptive and the confirmatory tests were not affected in the different supports, temperature, time, and storage conditions we used. As far as sensitivity is concerned, we found that the presumptive tests remained high, while confirmatory tests required samples which had not been subject to dilutions or washings.

Palavras-chave : Blood stains; Luminol; Phenolphtalein; Pyramidone; Takayama; agglutination inhibition; absorption-elution.

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