Archivos de Zootecnia
versão impressa ISSN 0004-0592
BARBOSA, A.P.M.; MARTINS, C.F. e SERENO, J.R.B.. Cryopreservation of bovine spermatogenic cells using different protective molecules. Arch. zootec. [online]. 2011, vol.60, n.230, pp.293-296. ISSN 0004-0592. http://dx.doi.org/10.4321/S0004-05922011000200014.
The objective of this study was to isolate mechanically bovine spermatogenic cells and assess their viability before and after cryopre-servation with three different cryoprotector molecules. The isolated cells presented an initial viability of 76.5%; after cryopreservation it was 51.7%, 55.5% and 58.8%, respectively for solutions with DMSO, propanodiol, and DMSO with propanodiol. The solution of DMSO with propanodiol was significantly better (p<0.05) than that with only DMSO, for preserve the cellular viability. Thus was possible to verify that spermatogenic cells isolated mechanically can be cryopreserved-thawed with larger viability than 55% in the solutions contends propanodiol. The cells frozen with the association of DMSO and propanodiol presented the largest integrity rate after the thawed, and can be applied in studies with ICSI.
Palavras-chave : Spermatid; ICSI; Cell preservation; Assisted reproduction.