Abstract

SMITH, O.F.; OGUNSOLA, A.A.; LADOKUN, A.O.  and  AJADI, T.A.. In vitro maturation of bovine granulosa cells for steroid hormone production. Arch. zootec. [online]. 2011, vol.60, n.232, pp.1331-1334. ISSN 1885-4494.  https://dx.doi.org/10.4321/S0004-05922011000400050.

This study aimed to evaluate the effects of preincubated granulosa cells on in vitro maturation and steroid hormones production in cows. Granulosa cells from the ovaries were cultured using the modified M-199 with Earle's salts. 1% bovine serum albumin was added and 5% fetal calf serum. Also 0.8% of antibiotics was also included. Cultures were incubated at 37^oC in a 95% air and 100% relative humidity. There were significant differences among values of progesterone and estradiol concentration obtained for levels of inclusion of FSH and LH. It can be concluded that there is necessity for supplementation of hormones in culture medium for bovine IVM.

Keywords : Cell culture; Culture medium.

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