SciELO - Scientific Electronic Library Online

vol.60 issue7 author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google


Archivos Españoles de Urología (Ed. impresa)

Print version ISSN 0004-0614


CARDONA MAYA, Wálter Darío; BERDUGO GUTIERREZ, Jesús Alfredo; RIOS, Jesús de los  and  CADAVID JARAMILLO, Ángela Patricia. Functional evaluation of sperm in colombian fertile men. Arch. Esp. Urol. [online]. 2007, vol.60, n.7, pp.827-831. ISSN 0004-0614.

Objective: The aim of the present study was to evaluate in 111 ejaculates from fertile men membrane integrity of spermatozoa before selection and sperm motility, and sperm concentration and chromatin integrity before and after selection of motile spermatozoa. Methods: We evaluated the membrane integrity (using hypoosmotic swelling test and Eosin-Y) before separation and chromatin integrity (using acridine orange), concentration and motility before and after separation by migration sedimentation technique. All individuals had pregnant wives or had procreated a baby during the last year. Results: The data of sperm membrane integrity by the eosin-Y and hypoosmotic swelling tests did not show signi ficant statistical differences and the correlation between them was low. The percentage of motile sperm (grades a + b) increased from 57% to 87% (p <0.001), the concentration decreased from 89 to 31 x 106 sperm/mL (p <0.001) and chromatin integrity increased significantly (p <0.0001) after separation of semen. Conclusions: The great variation in the values obtained in the functional test in fertile males requires a re-evaluation of the use of these tests in clinical practice of infertility.

Keywords : Spermatozoa; Chromatin; Eosin Y; Fertilization; Fertile men.

        · abstract in Spanish     · text in English     · English ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License