Abstract

LATORRE, A. et al. Estudio de correlación de Homocisteína plasmática por cromatografía  de intercambio iónico e inmunoanálisis de fluorescencia polarizada. Rev Diagn Biol [online]. 2001, vol.50, n.1, pp.33-37. ISSN 0034-7973.

The determination of plasma Homocysteine concentration is considered to be of particular interest for being as an independent risk factor for cardiovascular disease. Therefore, clinical laboratories should measure Homocysteine levels with reliable and rapid procedures in order to provide a correct approach to the biochemical factors playing a role in atherosclerosis.  Our laboratory developed an ion-exchange chromatography method based on the use of a post-column ninhydrin reaction, but this procedure was time consuming, which along with the increasing demand for the determination of total Homocysteine in plasma prompted us to evaluate a new automated fluorescence polarization inmunoanalysis.  We have developed and compared both methods. The within run and between run imprecisions ranged from 0.93 to 5.31%. The analytical recovery was 98.5 % for both methods. The detection limit study was 0.75 µmol/L and 0.79 µmol/L for the ion-exchange chromatography method and the fluorescence polarization inmunoanalysis respectively.  We found a significant relationship between the fluorescence polarization inmunoanalysis method and the ion-exchange chromatography method (y = 0.81 + 1.65x; r = 0.98).  In conclusion, the fluorescence polarization inmunoanalysis method is a reliable procedure that can be used for routine analysis of Homocysteine levels.

Keywords : Homocysteine; chromatography; inmunoanalysis.