Revista de Diagnóstico Biológico
versión impresa ISSN 0034-7973
ARANZADI, E. et al. Modificación de la técnica de determinación de mucopolisacáridos que utiliza azul de dimetilmetileno. Rev Diagn Biol [online]. 2002, vol.51, n.1, pp.13-15. ISSN 0034-7973.
One of the cationic colouring (dimethyl-metilene blue) techniques used in Clinical Laboratories for the screening of mucopolisaccharidosis recommends the GAG or mucopolisaccharides precipitation with CPC In this paper was introduced a modification that consisted in eliminating the precipitation phase in order to avoid any posible loss of sample which could take place in the consecutive decantations of the urines; moreover, it leads to a simplification of the technique. The results confirmed the initial hypothesis; a significant diference (p<0.01) between the measured urinary GAG in two confirmed mucopolysccharidosis (the highest levels) and a normal adult (the lowest level) was found. Within the medium levels, both techniques (precipitation and no precipitation) have identical results. In conclusion, the suggested modification not only simplifies the technique but also it makes it more reliable in the extreme urinary GAG levels.
Palabras clave : Mucopolisaccharides or glycosaminoglycans (GAG); cetylpiridinium (CPC); dimethyl-metilene blue (DMB); condroitine sulphate.