SciELO - Scientific Electronic Library Online

 
vol.27 issue4Relation of -55CT polymorphism of UCP3 gene with weight loss and metabolic changes after a high polyunsaturated fat diet in obese patientsGreen tea and its role on chemoprevention in vivo of genotoxic damage induced by carcinogenic metals (Chromium [VI]) author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google

Share


Nutrición Hospitalaria

On-line version ISSN 1699-5198Print version ISSN 0212-1611

Abstract

BETANZOS-CABRERA, G.; ESTRADA-LUNA, D.; BELEFANT-MILLER, H.  and  CANCINO-DIAZ, J. C.. Mice fed with a high fat diet show a decrease in the expression of "toll like receptor" (TLR)2 and TLR6 mRNAs in adipose and hepatic tissues. Nutr. Hosp. [online]. 2012, vol.27, n.4, pp.1196-1203. ISSN 1699-5198.  http://dx.doi.org/10.3305/nh.2012.27.4.5842.

Introduction: Pattern-recognition receptors (PRRs), which include Toll-like Receptor (TLRs) and Nacht leucinerich repeat proteins (NLRP/NALPs), are molecules of innate immunity able to recognize a wide variety of ligands present in microorganisms and human tissues. Adipocytes (fat cells) may play an important role in the physiological regulation of their own immune responses via TLRs. During obesity, the inflammatory pathway is triggered and insulin responsiveness is altered in fat tissue as a result of TLR4 activation by dietary lipids. Objective: Here, we investigate if other PRR family members could also participate in the inflammatory processes in the adipose tissue of obese mice. Methods: The mRNA expression of TLRs, the NLRP3-inflammasome (NLRP3, ASC, caspase-1 and IL-lbeta), IL-6, and TNFα in the hepatic and adipose tissues of mice fed with a high fat diet (HFD) were studied by RT-PCR. Results: Adipose tissue from mice fed with a HFD had decreased expression levels of TLR2, TLR6 and TLR7 and was similar to the pattern in hepatic tissue HFD mice. IL-6 and TNF-α expression also were decreased in adipose tissue of mice fed with a HFD. NLRP3-inflamma-some expression was not modified. Conclusion: These results suggest that the low expression of TLR2, and TLR6 in the mice fed with a HFD could be regulating the inflammation induced by the diet employed in this study.

Keywords : TLR; NLRP3; Adipose; Liver; Fat.

        · abstract in Spanish     · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License