Abstract

AKHTAR, Reyhan; ALI, Mohd; MAHMOOD, Safrannisa  and  NATH SANYAL, Sankar. Anti-proliferative action of silibinin on human colon adenomatous cancer HT-29 cells. Nutr. Hosp. [online]. 2014, vol.29, n.2, pp.388-392. ISSN 1699-5198.  https://dx.doi.org/10.3305/nh.2014.29.2.7080.

Background: Silibinin a flavonoid from milk thistle (Silybum marianum) exhibit a variety of pharmacological actions, including anti-proliferative and apoptotic activities against various types of cancers in intact animals and cancer cell lines. In the present study, the effect of silibinin on human colon cancer HT-29 cells was studied. Method: Incubations of cells with different silibinin concentrations (0.783-1,600 μg/ml) for 24, 48 or 72 h showed a progressive decline in cell viability. Results: Loss of cell viability was time dependent and optimum inhibition of cell growth (78%) was observed at 72 h. Under inverted microscope, the dead cells were seen as cell aggregates. IC50 (silibinin concentration killing 50% cells) values were 180, 110 and 40μg/ml at 24, 48 and 72 h respectively. Conclusion: These findings re-enforce the anticancer potential of silibinin, as reported earlier for various other cancer cell lines (Ramasamy and Agarwal (2008), Cancer Letters, 269: 352-62).

Keywords : Colon cancer; HT-29; Proliferation; Silibinin.

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