Resumen

LOYA MENDEZ, Yolanda et al. SNP-19 genotypic variants of CAPN 10 gene and its relation to diabetes mellitus type 2 in a population of Ciudad Juarez, Mexico. Nutr. Hosp. [online]. 2015, vol.31, n.2, pp.744-750. ISSN 1699-5198.  https://dx.doi.org/10.3305/nh.2015.31.2.7729.

Introduction: Diabetes Mellitus (DM) type 2 is a common pathology with multifactorial etiology, which exact genetic bases remain unknown. Some studies suggest that single nucleotides polymorphisms (SNPs) in the CAPN10 gene (Locus 2q37.3) could be associated with the development of this disease, including the insertion/deletion polymorphism SNP-19 (2R-3R). Objective: The present study determined the association between the SNP-19 and the risk of developing DM type 2 in Ciudad Juarez population. Methodology: For this study 107 participants were selected: 43 diabetics type 2 (cases) and 64 non diabetics with no family history of DM type 2 in first grade (control). Anthropometric studies were realized as well as lipids, lipoproteins and serum glucose biochemical profiles. The genotypification of SNP-19 was performed using peripheral blood lymphocytes DNA, polymerase chain reactions (PCR), and electrophoretic analysis in agarose gels. Once obtained the genotypic and allelic frequencies, the Hardy-Weinberg equilibrium test (GenAlEx 6.4) was also performed. Results: Using the X² analysis it was identified the genotypic differences between cases and control with higher frequency of the homozygous genotype 3R of SNP-19 in the cases group (0.418) compared to control group (0.265). Also, it was observed an association between genotype 2R/3R with elevated weight, body mass index, and waist and hip circumferences, but only in the diabetic group (P=< 0.05). Conclusion: The findings in this study suggest that SNP-19 in CAPN10 may participate in the development of DM type 2 in the studied population.

Palabras clave : Calpain-10; CAPN10 gene SNP-19; Diabetes Mellitus type 2; Candidate genes; Molecular marker.

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