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Nutrición Hospitalaria

On-line version ISSN 1699-5198Print version ISSN 0212-1611

Abstract

GARCIA, Cristina; MARIN, Clara; CATALA-GREGORI, Pablo  and  SORIANO, Jose Miguel. Use of bacteriphages against Salmonella enteritidis: a prevention tool. Nutr. Hosp. [online]. 2015, vol.31, n.6, pp.2740-2742. ISSN 1699-5198.  http://dx.doi.org/10.3305/nh.2015.31.6.8975.

Introduction: salmonellosis is a highly prevalent disease still searching for preventive tools to avoid contamination level priority public health. Objective: the in vitro effect of bacteriophages against Salmonella enteritidis was evaluated as a prevention tool. Method: two tests with three concentrations of bacteriophages were conducted against two strains of Salmonella Enteritidis inoculated in fresh faeces of laying hens. Each test had a positive control. Thus, four groups in each test were evaluated. Each experimental group included two replicates, and three plates were incubated per replicate. The concentrations tested were three: commercial solution (5 × 107 pfu/mL), and two dilutions (1/10 and 1/30). One of the strains tested was CECT 4300, a certified strain of Colección Española de Cultivo Tipo and the other a field isolated strain in a sacrificed hen farm. Both strains were inoculated at 1.3 × 105 cfu/g of faeces in each of the four groups. Isolation and identification of bacteria by ISO6579 was done at various times after inoculation: 1 minute, 24 hours and 7 days. Results: in the first test, with certified strain, Salmonella was isolated in all groups at time 1 minute. After 24 hours, Salmonella was isolated in all groups except in one of the replicas treated with 1/10 dilution of bacteriophages, one of the other replica plate treated with 1/10 dilution, and two plates of the two replicas treated with the commercial solution. After 7 days, the bacteria were not isolated from any of the experimental groups. In the second test, with the field strain, Salmonella was isolated in all groups at time 1 minute. After 24 hours, Salmonella was isolated in all groups except in one of the replicas treated with 1/10 dilution of bacteriophages and the two replicas treated with the commercial solution. Salmonella was not isolated in any of the experimental groups at 7 days. Conclusions: the use of bacteriophages reduced Salmonella enteritidis isolates in faeces at 24 hours after the application, so it could be considered as a prevention tool. At 7 days after inoculation of bacteria, no one was isolated in any of the experimental groups.

Keywords : Bacteriophage; In-vitro; Salmonella enteritidis; ISO6579.

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