SciELO - Scientific Electronic Library Online

 
vol.32 número4Manejo de la obesidad en el primer nivel de atención con un programa intensivo de cambio de estilo de vidaCaracterísticas sociodemográficas y sanitarias asociadas con bajo peso al nacer en Canarias índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Compartir


Nutrición Hospitalaria

versión On-line ISSN 1699-5198versión impresa ISSN 0212-1611

Resumen

ALVAREZ-SALA, Andrea; GARCIA-LLATAS, Guadalupe; BARBERA, Reyes  y  LAGARDA, María Jesús. Determination of cholesterol in human milk: an alternative to chromatographic methods. Nutr. Hosp. [online]. 2015, vol.32, n.4, pp.1535-1540. ISSN 1699-5198.  http://dx.doi.org/10.3305/nh.2015.32.4.9139.

Introduction: human milk (HM) is considered the best option for feeding healthy infants. Cholesterol (CHOL) is important for proper development of the nervous system, and for hormone and vitamin synthesis in growing infants. Breastfeeding and dietary CHOL intake during infancy have been suggested to affect blood lipid levels and the risk of cardiovascular disease in adulthood. Gas chromatography is the technique most widely used to determine CHOL in HM. Chromatographic methods are specific for the determination of CHOL and other sterols present in HM, but are extremely time consuming, and the costs and equipment requirements mean that they are not accessible to all laboratories. Aim: the present study describes the optimization and validation of an enzymatic-spectrophotometric method for CHOL determination in mature HM. Method: determination of CHOL involves fat extraction with chloroform:methanol, hot saponification and extraction of the unsaponifiable fraction with diethyl ether. CHOL was determined by an enzymatic method in which the concentration of the lutidine dye formed is stoichiometric to the amount of CHOL, and is measured by the increase in light absorbance at 405 nm. Results: human milk fat (mg/mL) (27.5 ± 1.3) and CHOL (0.113 ± 0.004) in analyzed HM were within the ranges reported by others authors. Analytical parameters of the proposed method were assessed: The precision values (%) (expressed as the relative standard deviation (RSD)) were: 3.5 and 6.7 for intra- and inter-day, respectively. Accuracy, estimated by recovery assays, was 110 ± 1.6%. Conclusion: the validated enzymatic-spectrophotometric method for determining CHOL in HM constitutes an alternative for fast and simple analysis of CHOL with equipment requirements accessible to any laboratory.

Palabras clave : Human milk; Cholesterol determination; Enzymatic-spectrophotometric method; Validation.

        · resumen en Español     · texto en Inglés     · Inglés ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons