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Avances en Odontoestomatología

versión On-line ISSN 2340-3152versión impresa ISSN 0213-1285


SIMANCAS-ESCORCIA, V; VERGARA HERNANDEZ, C  y  DIAZ-CABALLERO, A. Influence of bovine fetal serum in the culture of gingival fibroblasts. Av Odontoestomatol [online]. 2018, vol.34, n.6, pp.299-309. ISSN 2340-3152.


Gingival fibroblasts (FGs) are cells responsible for the maintenance of homeostasis, healing and immune tolerance of the gingival connective tissue. Due to their physiological characteristics, FGs can be a candidate in cell therapy. However, the conservation of their phenotype in cell culture requires strictly defined conditions. One of them is the concentration of Bovine Fetal Serum (FBS). Our aim is to evaluate the concentration-dependent effect of SFB supplementation in the culture medium on the behavior, growth, proliferation and survival of human gingival fibroblasts.

Materials and methods:

FGs were cultured with DMEM (Dulbecco's modification of Eagle medium) and concentrations of 0%, 0.5% and 10% of SFB for three weeks. Morphological, proliferation and immunohistochemical analyzes were carried out in the present work. A factorial analysis of variance (ANOVA) was carried out using the R Project software. The results were considered significant with a value of p <0.05.

Results and discussion:

The FGs grown with 10% FBS reached a more noticeable morphological development and in a shorter time compared to the FGs grown at 0.5% and 0% FBS. The effectiveness of the concentration of the SFB 0.5% was much higher in relation to the concentration of the SFB 10%. The immunodetention of actin, vimentin and fibronectin were more evident in the FGs treated with 10% and 0.5% FBS. This study concludes that human FGs have a better capacity for survival, development and proliferation when they are grown in the presence of FBS.

Palabras clave : Gingiva; Culture Media; Culture Media Serum-Free; Cell Proliferation; Immunohistochemistry.

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