Cirugía Plástica Ibero-Latinoamericana
versión impresa ISSN 0376-7892
ALMEIDA, K. A. et al. Stromal vascular fraction from fat tissue: obtaining stem cells and their yield according to the topography of the donor areas: previous note. Cir. plást. iberolatinoam. [online]. 2008, vol.34, n.1, pp.71-77. ISSN 0376-7892.
The harvest of adipose tissue will be a promising labor marketing for plastic surgeons, since tissue banks will certainly choose fat as the easiest way to obtain a high-yield source of stem cells, as this type of tissue can produce al least five times more colony-forming units (CFUs) than bone marrow extracts. The aim of this study is to show what can be expected from fat tissues as an origin of adult stromal vascular fraction (SVF) cells, and to evaluate the best areas to be elected as donor sites within the human body, all obtained by liposuction. The routine to obtain SVF cells by collagenase digestion of human adipose tissue samples was described. At the time of harvest, these cells displayed a viability of 92+/- 1% based on Trypan Blue exclusion, SVF cells were counted after 48 hours culture in Dulbecco ´s modified Eagle medium (DMEM) in a Neubauer counting chamber. The average yield of SVF cells was 7,2 +/- 1,3 x 103 cells per milliliter of liposuctioned tissue. As a conclusion, best strategies to obtain SVF cells are an important challenge nowadays. This study, although preliminary, showed that SVF may be easily obtained From liposuction. Comparison among different donor sites showed a 22% higher yield of SVF cells when fat tissue had been obtained from the trunk regions, when confronted with limbs.
Palabras clave : Stem cells; Fat; Adipose tissue; Liposuction; Lipectomy; Stromal vascular fraction.