versión impresa ISSN 1139-6709
MISHRA, Prashant K.; TEWARI, Jalpa P.; CLEAR, Randall M. y TURKINGTON, T. Kelly. Genetic diversity and recombination within populations of Fusarium pseudograminearum from western Canada. INT. MICROBIOL. [online]. 2006, vol.9, n.1, pp.65-68. ISSN 1139-6709.
Genetic diversity within populations of Fusarium pseudograminearum isolated from wheat grains from the Canadian provinces of Alberta and Saskatchewan was investigated. Three restriction enzymes (EcoRI, HaeIII, and PstI) were used to carry out restriction analysis of the nuclear ribosomal DNA (nrDNA) intergenic spacer region (IGS region) and eight primers were used to generate inter-simple sequence-repeat (ISSR) molecular markers. Our study indicated substantially high genetic diversity within these two populations, but low genetic differentiation and frequent gene flow among populations. The IGS data showed no genetic distinction between the two Alberta populations and only minor genetic differentiation between the Saskatchewan and Alberta populations. Analysis of molecular variance indicated that most genetic variability resulted from differences among isolates within populations. Multilocus linkage disequilibrium analysis suggested a panmictic population genetic structure and the occurrence of significant recombination in F. pseudograminearum. Regular gene flow and random mating between isolates from different populations could result in novel genotypes with both improved pathological and biological traits.
Palabras clave : Fusarium pseudograminearum; Gibberella coronicola; genetic diversity; gene flow; genetic recombination.