Meu SciELO
Serviços customizadosServiços Personalizados
Journal
Artigo
Espanhol (pdf)
Artigo em XML
Referências do artigo
Tradução automática
Enviar este artigo por emailIndicadores
Citado por SciELO 
Acessos
Links relacionados
Citado por Google 
Similares em
SciELO 
Similares em Google
Compartilhar
Permalink
Sanidad Militar
versão impressa ISSN 1887-8571
Resumo
ORTEGA GARCIA, M.ªV. et al. Quantitative PCR in real time to Burkholderia mallei ADN amplification: A comparison with the molecular method recommended by OIE. Sanid. Mil. [online]. 2017, vol.73, n.2, pp.85-90. ISSN 1887-8571. https://dx.doi.org/10.4321/s1887-85712017000200002.
Objective:
Comparison of two quantitative real-time PCRs for identification of Burkholderia mallei, on analytical sensitivity and specificity terms.
Methods:
Partial amplification of Burkholderia mallei gene: - orf11 and orf13 targeting the type III secretion TTS1 system cluster from Burkholderia genus by qPCR using hybridisation probes. - fliP targeting flagelin P from B. mallei by qPCR using TaqMan probe.Validity parameters determination.
Results:
The duplex assay developed by the Molecular Biology Laboratory at INTA obtained a limit of detection similar than that reached by the molecular method recommended by the OIE and permitted the specific amplification of B. mallei DNA.
Conclusions:
The duplex assay developed by the Molecular Biology Laboratory at INTA provides a rapid amplification of B. mallei DNA, also shows high analytical sensitivity and specificity. Furthermore, this assay permits the differentiation between B. mallei and B. pseudomallei.
Palavras-chave : Glanders; Burkholderia mallei; Quantitative real-time PCR; Sensitivity; Specificity.
