versão impressa ISSN 0211-6995
Nefrología (Madr.) vol.32 no.4 Madrid 2012
Catheter-related relapsing peritonitis due to Kocuria varians in a patient undergoing Continuous Ambulatory Peritoneal Dialysis
Kocuria varians is a Gram positive non-pathogenic commensal of the mammalian skin that can be present also in soil and water. Kocuria spp. was previously classified in the genus Micrococcus1 and identification of the species by automated systems has been reported to be problematic.2 Published cases of infections caused by Kocuria varians in the English literature are rare and consider patients with serious underlying disease.3 We report here a case of relapsing peritonitis in a patient undergoing Continuous Ambulatory Peritoneal Dialysis (CAPD) that was resolved after catheter removal.
The patient, a 70-year-old man with chronic renal failure and class III heart failure was admitted to the hospital on 26 July 2010 with turbid peritoneal dialysate, non febrile, having mild abdominal pain and positive Rebound sign at the physical examination. Leucocyte cell count showed the presence of 1200 leucocytes/μl (neutrophil count of 90%) in the peritoneal dialysate but subsequent culture of the fluid resulted negative. Treatment started with vancomycin 15mg/kg/5 days i.p. and aztreonam 2gr/d i.p. for a total of 20 days. On 30 July 2010 cell count was 50 leucocytes/μl and the patient was discharged.
3 days later however, at the first control visit, the peritoneal dialysate was turbid anew, the patient presented the same clinical findings and cell count revealed the presence of 300 leucocytes/μl. A Gram positive, spherical microorganism that occurred in tetrads with circular, smooth, glistering and yellow colonies was recovered from the peritoneal dialysate. The microorganism was identified as Kocuria varians by the VITEK 2 system. The isolate was susceptible to gentamycin, erythromycin, clindamycin, tetracycline, glycopeptides and linezolid while it was resistant to levofloxacin by the disc diffusion method. Following laboratory report, the patient was treated with vancomycin alone (15mg/kg/5 days i.p. for a total of 20 days).
Turbidity of the peritoneal dialysate did not reappear until 27 August 2010 when the patient was admitted to the hospital with generalized abdominal pain, positive Rebound sign and cell count of 550 leucocytes/μl. K. varians was isolated for the second time and removal of the peritoneal catheter followed by insertion of a new one in a different position was considered. Culture of the removed catheter was positive for K. varians. Vancomycin i.p. was administered, subsequent cultures were negative and the patient remained in good clinical condition since then.
Infections related to K. varians are uncommon but this species may act as opportunistic pathogen in immunocompromised patients with underlying diseases. Furthermore, K. varians is a biofilm forming bacterium4 thus probably complicating the antimicrobial treatment of catheter related infections. Erroneous identification of coagulase-negative Staphylococci as Kocuria spp. is possible and can be excluded with certainty only with the application of genotypic assays such as 16S RNA.1 In the present case the Vitek 2 system using the new GP identification card5 reported a "very good identification" for all three isolations. This case report aims on emphasizing the importance of careful consideration of the laboratory and clinical procedures when rarely pathogenic microorganisms are isolated in the peritoneal dialysate of patients undergoing CAPD.
Conflicts of interest
The authors declare that there is no conflict of interest associated with this manuscript.
Georgios Meletis1, Vasiliki Gogou1, Maria Palamouti1, Petros Spiropoulos2,
Kiriaki Xanthopoulou2, Paraskevi Tantou1, Artemisia Rizou1, Vasileios Thomoglou1
1Department of Clinical Microbiology. Veroia G. Hospital. Veroia (Greece)
2Nephrology Department. Veroia G. Hospital. Veroia (Greece)
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2. Ben-Ami R, Navon-Venezia S, Schwartz D, Schlezinger Y, Mekuzas Y, Carmeli Y. Erroneous reporting of coagulase-negative Staphylococci as Kocuria spp. by the Vitek 2 system. J Clin Microbiol 2005;43:1448-50. [ Links ]
3. Tsai CY, Su SH, Cheng YH, Chou YL, Tsai TH, Lieu AS. Kocuria varians infection associated with brain abscess: A case report. BMC Infect Dis 2010;10:102. [ Links ]
4. Midelet G, Kobilinsky A, Carpentier B. Construction and analysis of fractional multifactorial designs to study attachment strength and transfer of Listeria monocytogenes from pure or mixed biofilms after contact with a solid model food. Appl Environ Microbiol 2006;72:2313-21. [ Links ]
5. Boudewijns M, Vandeven J, Verhaegen J, Ben-Ami R, Carmeli Y. Vitek 2 automated identification system and Kocuria kristinae. J Clin Microbiol 2005;43:5832. [ Links ]
Department of Clinical Microbiology
Veroia G. Hospital, Veroia, Greece