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Nutrición Hospitalaria

On-line version ISSN 1699-5198Print version ISSN 0212-1611

Nutr. Hosp. vol.32 n.2 Madrid Aug. 2015

http://dx.doi.org/10.3305/nh.2015.32.2.8885 

ORIGINAL / Alimentos funcionales

 

Chemical composition, characterization of anthocyanins and antioxidant potential of Euterpe edulis fruits: applicability on genetic dyslipidemia and hepatic steatosis in mice

Composición química y caracterización de antocianinas y potencial antioxidante de la fruta Euterper edulis: aplicabilidad en la dislipidemia genética y la esteatosis hepática en ratones

 

 

Luciana Marques Cardoso1, Rômulo Dias Novaes2, Cynthia Aparecida de Castro3, Alexandre Azevedo Novello3, Reggiani Vilela Gonçalves4, Maria Esther Ricci-Silva5, Humberto Josué de Oliveira Ramos1, Maria do Carmo Gouveia Peluzio3 and João Paulo Viana Leite1

1Department of Biochemistry and Molecular Biology, Federal University of Viçosa.
2Department of Cell, Tissue and Developmental Biology, Federal University of Alfenas.
3Department of Nutrition and Health, Federal University of Viçosa.
4Department of Animal Biology, Federal University of Viçosa.
5Department of Vegetal Biology, Federal University of Viçosa, Brazil.

 

 


ABSTRACT

The significance of polyphenol intake for the prevention of chronic diseases is controversial.
Objective: this study investigated the chemical composition and antioxidant potential of an anthocyanin-rich extract from Euterpe edulis fruits (LPEF) and its effects on liver steatosis in dyslipidemic apoE-/- knockout mice.
Materials and methods: mice were divided into G1 (C57BL/6) standard diet; G2 (apoE-/-) standard diet, G3 (apoE-/-) 2% LPEF, G4 (apoE-/-) 6% LPEF, G5 (apoE-/-) 10% LPEF, G6 (apoE-/-) 2% α-tocopherol acetate. After 75 days of treatment, the animals were euthanized. The LPEF contained a high level of monomeric anthocyanins (301.4 mg/100g) and marked antioxidant activity.
Results: Catalase activity was reduced in G3, G4, G5 and G6 compared to G2. Superoxide dismutase was reduced only in G4. The animals in G4, G5, and G6 showed low HDL and triglycerides levels compared to G2. The proportion of lipid droplets in liver tissue was reduced in G4 and G5 compared to G2, G3, and G6.
Conclusion: The results indicated that E. edulis pulp is rich in anthocyanins and the LPEF dietary consumption can reduce the severity of liver steatosis in apoE-/- mice, an effect that is potentially mediated by the antioxidant activity of this extract and modulation of triglyceride serum levels.

Key words: Anthocyanins. Arecaceae. Euterpe edulis. Functional food. Liver disease.


RESUMEN

El papel de los polifenoles en la prevencion de enfermedades cronicas es controvertido.
Objetivo: este estudio investigo la composicion quimica y el potencial antioxidante de un extracto del fruto de Euterpe edulis rico en antocianinas (LPEF) y sus efectos en la esteatosis hepatica en ratones apoE-/- knockout con dislipidemia.
Material y metodos: los ratones fueron divididos en los siguientes grupos; G1 (C57BL/6) con una dieta estandar; G2 (apoE-/-) con dieta estandar; G3 G3 (apoE-/-) con 2% de LPEF; G4 (apoE-/-) con 6% de LPEF; G5 (apoE-/-) con 10% de LPEF y G6 (apoE-/-) con 2% acetato α-tocoferol (α-tocopherol acetate). Despues de 75 dias de tratamiento, los animales fueron eutanizados. El LPEF contenia un alto nivel de antocianinas monomericas (301,4 mg/100 g) con notable actividad antioxidante.
Resultados: la actividad catalasa fue reducida en los grupos G3, G4, G5 y G6 comparada con G2. La superoxidasa dismutasa solo se redujo en el grupo G4. Los animales de G4, G5 y G6 mostraron bajos niveles de HDL trigliceridos, comparados con G2. La proporcion de lipidos en el tejido hepatico fue reducida en G4 y G5, comparado con G2, G3 y G6.
Conclusion: los resultados indicaron que la pulpa de E. edulis es rica en antocianinas, y que el consumo de LPEF en la dieta puede reducir la severidad de la esteatosis hepatica en ratones apoE-/-, un efecto que es potencialmente mediado por la actividad antioxidante de este extracto y la modulacion en los niveles sericos de trigliceridos.

Palabras clave: Antocianinas. Arecaceae. Euterpe edulis. Alimentacion funcional. Enfermedad hepática.


 

 

http://scielo.isciii.es/pdf/nh/v32n2/30originalalimentosfuncionales02.pdf

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