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versión On-line ISSN 1698-6946
Med. oral patol. oral cir.bucal (Internet) vol.12 no.2 mar. 2007
Roberto López-Píriz1, Lorenzo Aguilar2, Maria José Giménez3
(1) Degree in Medicine and Surgery. Degree in Odontology. Doctoral student
(2) Doctor in Medicine and Surgery
(3) Doctor in Pharmacy. Microbiology Department, Faculty of Medicine, UCM
The dental biofilm is a complex bacterial ecosystem that undergoes evolution, maturing and development, and thus leads to odontogenic infection. The infection is normally located in the tissues of the dental organ itself, and follows a chronic course of evolution. However, bacterial pathogens express virulence factors in the biofilm, and this together with changes in host immunity, may cause clinical exacerbations and spread of infection to other areas of the body. Odontogenic infection management should take into consideration the fact that therapeutic success lies in the control of the infectious aetiologic agent, using mechanical-surgical debridement and/or antimicrobial therapy. Debridement techniques have a fundamentally quantitative effect (by reducing the size of the inoculum) and therefore if these techniques are used alone to control infection, despite an initial clinical improvement that is sometimes prematurely considered as therapeutic success, odontopathogens may persist and the process may recur or become chronic. Microbiological examination may be helpful in defining therapeutic success in a more reliable way, it could define the prognosis of recurrence more precisely, and could enable the most appropriate antibiotic to be selected, thus increasing therapeutic efficacy. Antimicrobial therapy brings about a quantitative and qualitative change in the bacterial composition of the biofilm, in addition to being able to act on sites that are inaccessible through mechanical debridement. However, incorrect antimicrobial use can lead to a selection of resistant bacterial species in the biofilm, in addition to side effects and ecological alterations in the host. In order to minimise this risk, and obtain maximum antimicrobial effect, we need to know in which clinical situations their use is indicated, and the efficacy of different antibiotics with regard to bacteria isolated in odontogenic infection.
Key words: Odontogenic infection, treatment, odontogenic infection, microbiological diagnosis, dental biofilm, dental abscess.
La biopelícula dental es un ecosistema bacteriano complejo cuya evolución, maduración y desarrollo ocasiona la infección odontogénica. Habitualmente la infección se encuentra localizada en los tejidos del propio órgano dentario y sigue un curso evolutivo crónico, aunque la expresión de factores de virulencia bacterianos en la biopelícula o cambios en la situación inmune del huésped pueden ocasionar exacerbaciones clínicas y la extensión de la infección a otras zonas del organismo. En el abordaje de la infección odontogénica debemos tener presente que el éxito terapéutico radica en el control del agente etiológico infeccioso, mediante desbridamiento con técnicas mecánico-quirúrgicas y/o administración de antimicrobianos. El efecto de las técnicas de desbridamiento sobre la biopelícula es fundamentalmente de tipo cuantitativo (disminuye el tamaño del inóculo) de modo que su empleo como único medio de control de la infección puede suponer la persistencia de odontopatógenos y la recurrencia o cronificación del proceso, aunque obtengamos una mejoría clínica inicial que en ocasiones consideramos éxito terapéutico de modo precipitado. Realizar un estudio microbiológico podría ayudar a definir el éxito terapéutico con mayor fiabilidad, dar más exactitud a nuestros pronósticos de recurrencia, y seleccionar el antibiótico más apropiado aumentando la eficacia terapéutica. El tratamiento antimicrobiano ocasiona un cambio cuantitativo y cualitativo en la composición bacteriana de la biopelícula, además de poder actuar en lugares no accesibles al desbridamiento mecánico. Sin embargo, un mal uso de los antimicrobianos puede ocasionar selección de especies bacterianas resistentes en la biopelícula además de efectos secundarios y alteraciones ecológicas en el huésped. Para minimizar este riesgo y obtener el máximo rendimiento del empleo de antimicrobianos es preciso conocer las situaciones clínicas que indican su uso y la eficacia de los distintos antibióticos frente a los aislados en la infección odontogénica.
Palabras clave: Infección odontogénica/tratamiento, infección odontogénica, diagnóstico microbiológico, biopelícula dental, absceso dental.
Odontogenic infection is the most prevalent disease worldwide and it is the principal reason for seeking dental care. The commonest emergency odontogenic infections are periapical abscess (25%), pericoronitis (11%) and periodontal abscess (7%). Its significance in health problems is also reflected by the fact that 12% of antibiotics are prescribed for odontological reasons. (1)
Aetiology of odontogenic infection
The World Health Organisation accepts that the dental biofilm is the aetiological agent of odontogenic infection, and defines biofilm as a proliferative bacterial, enzyme-active ecosystem. As soon as a baby is born, a colonisation process commences and this results in the development of a pioneer community in the mouth with predominance of Streptococcus salivarius. At the age of six months (when the first teeth appear) this community has a majority presence of S. sanguis and S. mutans, and by the time dentition has been completed, there is a heterogeneous aerobic and anaerobic community. It is estimated that up to 700 species may colonise the mouth, 400 of which colonise the subgingival area. Biofilm evolution is an autogenic succession process based on bacterial interactions that occur through physical contact, metabolic exchange, communication through signalling (quorum sensing) and genetic material exchange. Coaggregation is based on specific recognition of different bacterial species and one of the principle mechanisms of evolution and maintenance of the biofilm. (2)
Odontogenic infection is polymicrobial and mixed. It is the result of "biofilm maturing" that consists of a change in the predominant bacterial species (from predominantly gram-positive, facultative and saccharolytic flora to predominantly gram-negative, anaerobic and proteolytic flora), the association of different bacterial morphotypes and increased bacterial diversity. Fusobacterium nucleatum is considered as the central structural component of biofilm because it coaggregates with the biofilm components that do not cause disease and with periodontal pathogens, thus permitting biofilm evolution into odontogenic infection.
In 1992, Socransky modified Kochs postulates, laying down criteria for identifying periodontal pathogens. (3) Biofilm study using DNA hybridisation techniques enabled detection of associations of specific bacterial species and their relation with health or disease. "Purple" (with fundamentally aerobic and immobile flora), "yellow" and "green" complexes are not associated with disease, whilst orange (F. nucleatum/periodonticum, P. intermedia, P. micros) and red (P. gingivalis, T. forsythia, T. denticola) complexes cause disease in the presence of virulent clones with intra- and extrachromosomic genetic information in a sufficient quantity to overcome the immune resistance of the host. A. actinomycetemcomitans is also considered as a periodontal pathogen although it is not included in any particular group.
Pathogenics of odontogenic infection
The supragingival biofilm is fundamentally gram-positive, facultative and saccharolytic, which means that in the presence of sugars, it produces acids that demineralise enamel, facilitating biofilm infiltration of dentin and pulp. With the bacterial invasion of the tooths internal tissue, the biofilm evolves, and thus root canals are infected with predominantly gram-negative, anaerobic and proteolytic bacteria. Some of these bacteria have virulence factors that enable them to invade periapical tissues through the apical foramen. (3) More than half of active periapical lesions cannot be detected on X-ray because they measure less than 0.1 mm2. (4) If the host immune response causes an accumulation of neutrophils, this will cause a periapical abscess, which is a destructive tissue lesion. But if the response is predominantly mediated by macrophages and T-cells, an apical granuloma will develop, characterised more by tissue reorganisation than by tissue destruction. Changes in host immunity or in bacterial virulence may cause reactivation of silent periapical lesions.
Odontogenic infection may also originate in periodontal tissues. When the subgingival biofilm evolves and incorporates periodontal pathogens that express virulence factors, this triggers an immune response in the host causing damage tending to chronicity that finally causes periodontal bone loss. The periodontal abscess may originate from an exacerbation of chronic periodontitis, the presence of dental defects that facilitate bacterial invasion (e.g. development grooves, root fusion, etc.) or it may be of iatrogenic origin, due to impaction of a calculus in the epithelium of the periodontal pocket during scaling or incorrect debridement that only solves gingival distension at a coronal level, preventing drainage of apical zone of the pocket. (5)
Some abscesses form a fistula and become chronic. They are generally asymptomatic or paucisymptomatic. A special form of recurrent periodontal abscess is pericoronitis, caused by bacterial invasion of the coronal pouch during molar eruption.
It is sometimes unclear as to whether the infection has a periodontal or pulpar origin, and therefore the term endoperiodontal lesions are used. (4) There is some controversy as to whether a pulpar lesion can cause periodontal damage and vice versa, which raises several questions at a clinical level: should we perform prophylactic canal treatment of teeth with moderate or advanced periodontitis? Should a tooth with pulpar necrosis be replaced by an implant in order to prevent periodontal bone loss? The pulp can be invaded from the periodontium through dentin tubules, lateral canals and the apical foramen, but it is very rare to find a tooth without pulp vitality and free of caries, restoration work or trauma to justify it, and therefore periodontal disease and periodontal mechanical debridement do not appear to have a significant influence on pulp vitality. Teeth without pulp vitality and with active periapical lesions present greater marginal bone loss / year than teeth with pulp vitality, but these differences are not significant enough in periodontal prognosis to justify preventive tooth extraction. Pulpar infection can form a fistula in the periodontium, causing a deep and narrow periodontal lesion. In general, the presence of pulp vitality indicates that the bacterial invasion has occurred from or through the periodontium, and the absence of pulp vitality suggests an endodontic origin of infection. If an in-depth clinical examination is carried out, few lesions will actually be classified as endoperiodontal lesions.
Diagnosis of odontogenic infection
Diagnosing the origin of odontogenic infection is fundamental in order to manage treatment appropriately. The problem tooth needs to be identified, as well as the tissue where the biofilm bacteria have commenced their invasion. Clearly, a thorough physical examination is required, along with X-rays and pulp vitality tests.
Clinical manifestations may be useful when they are referred to a specific tooth (e.g. periodontal abscess), but manifestations are often diffuse or even refer to areas that are adjacent to the origin of the infection (e.g. pulpitis). Odontogenic infection may also occur without symptoms (e.g. chronic periodontitis) or with very few symptoms (e.g. periapical abscess that has formed a fistula), and this could only be manifested on clinical examination. A clinical examination consisting in inspection and percussion of teeth is essential to identify the origin of infection.
X-rays provide essential information but certain limitations should be considered: 1. Some periapical cysts may be larger than a periapical X-ray film, and therefore only the contents will be observed; 2. During early stages of infection changes in bone density may not be noticeable; 3. Only bidimensional information can be gained, and therefore fractures that are perpendicular or oblique to the X-ray may not be detected.
In addition, pulp vitality tests help to identify target tooth and also the likely origin of bacterial invasion: infection may be of pulpar origin if the test is negative or if there is an exaggerated response to stimulus; it may be of periodontal origin if positive test without discrepancies with that of other teeth.
However, there is some controversy regarding the utility and indication of microbiology diagnostic tests in odontogenic infections. Clearly there is no need to perform a microbiology examination in order to diagnose periodontitis, but a microbiological analysis of the infection may be useful if results enable us to improve our therapeutic strategies and ensure successful treatment, and in any case it serves to provide epidemiological data. (6)
Microbiological analysis can help to identify the origin of infection. Although all odontogenic infections are result of biofilm evolution, and there are therefore marked similarities in the bacterial composition of odontogenic infections, regardless of their tissue of origin (Table 1), some bacterial species are specific to abscesses with pulpar origin (e.g. Porphiromonas endodontalis); while others will rule out this origin (e.g. A. actinomycetemcomitans). (7,8)
However, odontological microbiological studies are complex to perform. Sampling is a difficult task since samples often become contaminated, thus altering results. Samples must be transported in appropriate media for mixed flora.
Differences in bacterial identification methods are also the cause of discrepancy in conclusions drawn by different studies that have analysed the bacterial composition of odontogenic infections. Bacterial culture is the traditional method of identification, but some bacteria require special culture methods (e.g. T. forsythia) and others can only be identified using immunofluorescent techniques or DNA hybridisation. (6)
Keyes used differential darkfield microscopy to determine the level of spirochetes and motile rods, thus ending treatment when there was a reduced number found on the biofilm examined. (6) It is now known that some bacteria behave as exogeneous pathogens and some bacterial associations related to a low treatment response or high rate of recurrence. (3) For example, the presence of A. actinomycetemcomitans; P. gingivalis; or P. intermedia is associated with recurrence in periodontal disease activity. Thus, since the microbiological examination can identify odontopathogens and determine their numbers in the biofilm, then it can help to make decisions on a specific therapeutic strategy and to monitor results of clinical studies. In short, microbiology helps to define therapeutic success and the risk of relapse more precisely, thus avoiding a prognosis based exclusively on clinical data (i.e. initial improvement following debridement).
Microbiological cultures also allow the study of susceptibility of odontopathogenic isolates to different antibiotics. They can determine which antimicrobial agents are the most effective in odontogenic infections, by determining minimum inhibitory concentrations. These data are the basis of studies relating odontopathogenic susceptibility and antimicrobial pharmacokinetics/pharmacodynamics, in order to establish the most effective therapeutic regimen.
Treatment of odontogenic infection
The objective of antibacterial treatment is to control (reduce or eliminate) the infective bacterial load. To achieve this, in the case of odontogenic infection, therapeutic action combines mechanical debridement, and/or surgery, and/or systemic antibiotic therapy, where appropriate.
The first step in the case of dental abscess is to drain and debride the abscess using mechanical-surgical techniques. Drainage is performed by making an incision in the area of greatest fluctuation. If the abscess is of an endodontic origin, drainage can be performed through the root canals. Tooth extraction provides a drainage route and eliminates the entrance route for the infection, but it is only indicated in the acute phase after having balanced these benefits against the risk of spreading bacterial inoculum during surgery. Tooth extractions that require flap surgery or ostectomy should be delayed until a second stage when infection has been controlled. Mechanical debridement eliminates necrotic tissue and bacterial residues, and consists in debridement of the root surface in the case of periodontal involvement, or of the bone canal in the case of pulpar infection.
Mechanical-surgical techniques have a quantitative effect on bacterial load giving the host the opportunity to recover homeostasis through immune system action. (9) However, these techniques do not modify the composition of the biofilm and the persistence of odontopathogens that could lead to recurrence or a chronic state. A. actinomycetemcomitans and P. gingivalis cannot be eliminated in the majority of deep periodontal lesions with root debridement alone. (7) The persistence of S. faecalis in root canals is related to the need to repeat endodontic treatment, and the percentage of failed root canal treatment according to X-ray results is estimated at about 50%. (4,8) Furthermore, some bacteria (Actinomyces israelii; Propionibacterium propionicum) are capable of reaching the dental periapex and remain there, requiring an apicectomy in an attempt to eliminate persistent infection.
Additional techniques to complement mechanical treatment include chemical debridement by means of topical application of antiseptics and antimicrobial agents. It is recommended to use antiseptic irrigation concomitantly during mechanical debridement, but the use of topical substances is not indicated during the acute phase because they hinder drainage. (5) Root canals can be filled with antiseptic substances for a long period of time, but such substances have little or no action at a periapical level. The topical use of antimicrobials should be restricted because this favours the development of resistance and their clinical effect is limited to the application surface since they do not act on invasive bacteria. (4,5,7)
The relevance of antimicrobials in the management of odontogenic infection lies in their clinical utility when administered systemically. Systemic antibiotic therapy prevents the infection from spreading and it acts in places that mechanical treatment cannot reach. It therefore act more specifically on odontopathogens than debridement does. (10) It is indicated for therapeutic purposes in fast-progressing and recurrent periodontitis, and in all odontogenic infections with rapidly evolving signs and symptoms (24-48 hours), trismus, oedema, and/or systemic manifestations such as malaise, fever, tachypnoea, dyspnoea, cellulites, lymphadenopathy and hypotension. (11) Systemic antibiotic therapy is also indicated in the prophylaxis of systemic or distant infection and as a preventive treatment of infection in immunodepressed patients or in patients with severe systemic disease undergoing an invasive or long time dental procedure. (10,12,13)
It has been demonstrated that periodontal abscesses have resolved solely by means of systemic antibiotic therapy. (5,14,15) However, debridement should be the first step in therapy because draining the infection and eliminating necrotic waste will facilitate antimicrobial action. Treatment with antimicrobials alone is indicated when the severity of the infection advises delaying surgical techniques due to the risk of spreading the infection during debridement itself. (11) Antibiotic prophylaxis achieves better results if the antimicrobial agent is administered pre-operatively. (10,13)
Paradoxically, treatment of chronic asymptomatic infection can cause an acute exacerbation of the infectious process. Periodontal scaling and endodontic over-instrumentation can cause bleeding and exudates in periodontal and periapical tissues, providing bacteria with nutrients, and thus stimulating proliferation, which may overcome the hosts immune resistance. This factor should be given special consideration in immunodepressed patients and in patients whose microbiological studies reveal odontopathogens or bacterial associations that are particularly resistant to therapy. In these cases, it is essential to use an effective antimicrobial agent. (10,13)
Only physicians or dentists should select which antimicrobial agent to use, because it is a complex clinical process that is based on empirical evidence and clinical, microbiological and pharmacokinetic knowledge. (10)
The use of an antibiotic with an inappropriate spectrum that does not act on certain resistant bacterial species will lead to excess proliferation of the latter by eliminating other sensitive species. This may trigger acute exacerbations or persistent infection. Wide spectrum antibiotics must be used in view of the polymicrobial, mixed nature of odontogenic infections, and such antibiotics must be especially active against the commonest odontopathogens. When selecting an antibiotic, consideration should be made for natural resistance (e.g. Strepococcus sp.; Actinomyces sp. and A. acinomycetemcomitans against metronidazole) and the possible presence of acquired resistance, which are those that take advantage of incorrect antibiotic use.
Some studies have analysed the susceptibility of bacterial species in the biofilm to antibiotics in Spain. (16)
These studies show a high rate of resistance to certain antimicrobial agents and beta-lactamase production by Prevotella spp, Fusobacterium spp.; Capnocytophaga spp. and some strains of Veionella spp.(17-19). From results obtained (Table 2), there is consensus regarding the treatment and prophylaxis of odontogenic infection in which amoxicillin associated with clavulanic acid is indicated as the first choice antibiotic, and clindamycin as an alternative in the event of penicillin allergy. (12,13,20)
Antimicrobial pharmacodynamics determine the most effective dosage regimen to attain bacterial eradication without encouraging the development of resistance. The most commonly used antibiotics in odontology are time-dependent, which means that to be effective, their physiological levels must exceed the minimum inhibitory concentration for at least 40% of the time between doses. (16) Taking into account the susceptibility of isolated bacteria, resistance mechanisms and antibiotic pharmacodynamics, high doses of amoxicillin / clavulanic acid (875/125 mg three times daily or 2000/125 mg twice daily) are the most appropriate treatment of caries-associated odontogenic infections (pulpitis, abscesses), and of periodontal infections when so required, and clindamycin provides an alternative choice, at a dose of 600 mg three times daily.
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Dr. Roberto López-Píriz.
C/ General Álvarez de Castro, nº 5, 1º B.