29 10 
Home Page  

  • SciELO

  • Google
  • SciELO
  • Google


Actas Urológicas Españolas

 ISSN 0210-4806

PRIETO VILLAPUN, J.C.; SOLANO HARO, R.M.; CARMENA SIERRA, M.J.    SANCHEZ-CHAPADO, M.. Importance of  heterotrimeric G proteins in prostate cancer molecular biology. []. , 29, 10, pp.948-954. ISSN 0210-4806.

Objective: To deep in the knowledge of the involvement of G-protein αs and αi subunits in human prostate cancer. Methods: Prostate tissue from 9 patients undergoing radical prostatectomy for prostate cancer and 5 controls undergoing cystoprostatectomy for bladder carcinoma. G-protein αs and αi subunits were studied for expression (mRNA by RT-PCR and protein by Western-blot), functionality (adenylyl cyclase activity, AC) and possibility of mutations (analysis with restriction enzymes and cDNA sequentiation). Results: At mRNA level, the expression of αs, αi1, αi2 y αi3 was detected in healthy and cancerous tissues. At protein level, the expression of αs y αi1,2 diminished (25% and 40%, respectively) in prostate cancer. The expression of αi3/0 also diminished, whereas that of ί subunit was not modified. Basal AC activity in adenocarcinoma membranes was 40% inferior to the control. Digestion with restriction enzymes Eag I or AlwN I did not allow to locate mutations in αs. However, digestion at αi2 level with BstU I enzyme served to observe a change of Gln205 (CAG triplet) to Pro (CCG). Conclusions: The functionality and expression of heterotrimeric G proteins are selectively modified in human prostate adenocarcinoma, occurring in addition some punctual mutation. The observed substitution of Gln205 by Pro may result in a low GTPase activity for αi2 that, therefore, is stabilized in its active form.

: Prostate cancer; G proteins; Expression; Mutations.

        · |     · |     · ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License