Abstract

LUPCHINSKI JR., E. et al. Genetic characterization of three Nile tilapia (Oreochromis niloticus) strains. Arch. zootec. [online]. 2011, vol.60, n.232, pp.985-995. ISSN 1885-4494.  https://dx.doi.org/10.4321/S0004-05922011000400015.

The aim of this study was to analyze the genetic diversity of three Nile tilapia (Oreochromis niloticus) strains, through the RAPD markers. Ninety adult individuals (30 of each strain) of two fish farms stations located in the Maringá (Bouaké - B and GIFT - G strains) and Guaíra (Chitralada C strain) cities, in the Paraná State (Brazil) were analyzed. The 13 selected primers yielded 72 fragments of which 60 (83.3%) were polymorphic. Differences (p<0.05) in the frequency of 33 fragments were observed, with 14 eliminated. The genetic variability results estimated by the percentage of polymorphic fragments and for the genetic diversity of Shannon index indicated a high intra-populational genetic variability. According with the AMOVA, most of the variation is within each strain. This result was corroborated with the FST values that showed a moderate genetic differentiation. It was also verified that C and G were the strains but similar genetically and that B and G presented less genes in common. The results of this study facilitated the correct reproductive and genetic management of these fish strains.

Keywords : Bouaké; Chitralada; Genetic variability; GIFT; RAPD.

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