Abstract

SILVA, S.C.C. et al. Allele and genotype frequency of DGAT1 gene in a cattle population. Arch. zootec. [online]. 2011, vol.60, n.232, pp.1343-1346. ISSN 1885-4494.  https://dx.doi.org/10.4321/S0004-05922011000400053.

DGAT1 gene is a candidate gene involved in fat content in milk and. This study analyzed DNA samples from 72 dairy cattle using blood samples. DNA was extracted use CTAB and proteinase K. The target region of the gene was amplified by PCR and the results were visualized with the use of polyacrylamide gel. The level of significance adopted was 5% for all analysis. Four alleles were checked their frequencies were: A= 4.0%, B= 50%, C= 40%, and D= 6.0%. It was verified the Hardy-Weinberg using the Chi square test concluding that the population was not in balance.

Keywords : Diacylglycerol acyltransferase 1; Polymorphism; Fat synthesis.

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