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Actas Urológicas Españolas
Print version ISSN 0210-4806
Abstract
FATELA-CANTILLO, D. et al. Influence of the delay in urinary NMP22 preanalytical processing. Actas Urol Esp [online]. 2007, vol.31, n.7, pp.746-751. ISSN 0210-4806.
Objectives: To evaluate the influence of the delay in urinary NMP22 preanalytical processing. Material and methods: Twenty-eight voided urine samples were taken: bladder cancer (14), urine tract infections (4), lithiasis (4), healthy volunteers (1), and with other no malignant bladder diseases (5). All samples, were maintained at environment temperature, and were processed according to the stabilization of parts of urine collected at 0, 30, 90 and 150 minutes. Samples were stored at 4 ºC until its determination. NMP22 was determined with the IMMULITE One analyzer. Results: There were no significant differences for NMP22 levels between each different point of time studied. Conclusions: Delay up to 2 hours and a half when we add stabilization solution to urine samples no affects NMP22 results. Thatthing, might provide more confidence and flexibility on quantitative immunoassays that required urine stabilization.
Keywords : Bladder cancer; Urine; Tumour marker; NMP22; Preanalytical phase.