Abstract

ALVAREZ-VIJANDE, R.; LUQUE GALVEZ, P.; ALCARAZ ASENSIO, A.  and  GRUPO DE TRASPLANTE RENAL EXPERIMENTAL. Cell energetic loading in experimental renal transplant uit different periods of warm ischemia. Actas Urol Esp [online]. 2008, vol.32, n.1, pp.41-58. ISSN 0210-4806.

Introduction and goals: Renal procurement after a period of heart stop demands a previous knowledge of ischemia-reperfusion injuries means. To study cell injury mechanisms an experimental study has been designed in pigs, with different rangres of warm ischemia (0-30-45 and 90 min). The main goal was to research on the basis of ischemic injury. Material and methods: Biochemical parameters (creatinine, urine output), energetic loading (ATP, ADP, AMP and global energetic loading) and pathological studies as long as survival analysis by 5th day were completed. Results: Animal survival and graft viability range from 100% at 5th day in control and 30 min warm ischemia groups to 60% in 90 min warm ischemia group. Creatinine levels rises at 1st, 3rd and 5th day, especially in those non-viable organs. ATP levels decrease after warm ischemia period, increases ADP and AMP levels after reperfusion in those viable organs. Conclusions. Prolonged periods of warm ischemia do not result necessarily in non-viable kidneys. Viable organs recover nucleotide levels early. Study of energetic cell loading levels is a good way to get on better in the knowledge of injury mechanisms after ischemiareperfusion.

Keywords : Warm ischemia; Energetic resource; Non heart beating organ donor.

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