Abstract

CORTES-CASTELL, Ernesto et al. Protection by polyphenol extract from olive stones against apoptosis produced by oxidative stress in human neuroblastoma cells. Nutr. Hosp. [online]. 2016, vol.33, n.1, pp.118-122. ISSN 1699-5198.  https://dx.doi.org/10.20960/nh.39.

Objective: We evaluated the protective activity of an extract from a by-product such as olive stones, through its ability to inhibit H₂O₂ induced apoptosis in the SH-SY5Y human neuroblastoma cell line. Material and methods: To such end, 20,000 cells/well were cultivated and differentiation with retinoic acid was initiated. Once the cells were differentiated, apoptosis was induced with and without H₂O₂ extract. Finally, cDNA extraction was performed, and pro-apoptotic genes Bax and anti-apoptotic genes Bcl-2 were analyzed. Quantification of the gene expression was performed using the GAPDH gene marker. Results: Cell viability with the extract is 97.6% (SD 5.7) with 10 mg/l and 62.8% (SD 1.2) to 50 mg/l, using 10 mg/l for the biomarker assay. The retinoic acid differentiated SH-S cell line (10 µM) shows a clear apoptosis when treated with H₂O₂ 150 µM, with a Bax/Bcl-2 ratio of 3.75 (SD 0.80) in contrast to the differentiated control cells subjected to H₂O₂ and with extract, which have the same ratio of 1.02 (SD 0.01-0.03). Conclusion: The olive stone extract shows anti-apoptotic activity in the provoked cell death of SH-SY5Y human neuroblastoma cells in their normal state, defending them from oxidative stress which produces a significant increase in the apoptotic gene ratio in contrast to anti-apoptotic genes (Bax/Bcl-2).

Keywords : Polyphenols; Olive stones; Apoptosis; Human neuroblastome cells.

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