Abstract

ESPARZA ITURBIDE, R. et al. Autologous mesothelial cells transplantation as accelerator and skin healing modifier in rats. Cir. plást. iberolatinoam. [online]. 2013, vol.39, n.1, pp.47-51. ISSN 1989-2055.  https://dx.doi.org/10.4321/S0376-78922013000100006.

The purpose of this study was to verify if the autologous peritoneal mesothelial cells in full thickness wounds on rats, speed up and adjust the normal skin healing process. Based on the theory that mesothelial cells from tissues such as peritoneum, pleura or pericardium, are responsible for one of the faster healing process and synthesize stimulating wound healing and chemotactical factors (hence the genesis of surgical adhesions), besides possessing the ability to differentiate into other cell series (plasticity). We designed a pilot, analytical, longitudinal, prospective and comparative study in the Laboratory of Experimental Surgery at The American British Cowdray Medical Center, Mexico City (México). Were used 15 Wistar rats which were divided into 2 groups: Group I (n = 5) where after general anesthesia, skin removed 3 mm in diameter with microsurgical technique in the back and was close by secondary intention; and Group II or experimental group (n = 10) where laparotomy was performed with excision of the parietal peritoneum and primary closure, excision of full thickness skin on the dorsal surface of 3mm diameter and peritoneal autograft placement on the dorsal wound. In histological analysis, were reviewed 6 variables: collagen, fibroblasts, number of vessels, macrophages, inflammatory cells and retraction, to point out fully the nature and characteristics of healing in both groups. For the statistical analysis we used Statistical Package for Social Sciences 17.0. The descriptive statistics was made using frequency measures of central tendency and dispersion. The results showed that the Group I rats, had increased inflammation, fibrosis and retraction, data support a proliferative phase of healing. In the Group II or experimental were found less inflammation and fibrosis, increased collagen and data consistent with a remodeling phase. In conclusion, we found that autologous peritoneal mesothelial cells in full thickness wounds accelerates the normal skin healing in rats by decreasing inflammation, fibrosis and increased collagen.

Keywords : Healing; Transplantation; Mesothelial cells.

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