Background and Objective:

Adipose tissue is an easy access source of stromal cells. For storage and subsequent clinical application, it is important that the method of cryopreservation used maintain adequate viability rates after a cycle of freezing.

Our aim is to get stromal cells derived from human adipose tissue, implement a protocol of cryopreservation free of animal protein and subsequent measure viability rates after a cryopreservation cycle for its posterior clinical use.

Methods:

Fresh adipose tissue of 5 patients undergoing liposuction was used and stromal cells were isolated by enzymatic digestion techniques and cell expansion. Characterization of the cells was performed by immunophenotyping. Cells using 10% dimethylsulfoxide and stored for 1 month were cryopreserved in liquid nitrogen. Viability rates were assessed by propidium iodide and flow cytometry before and after a cryopreservation cycle.

Results:

Stromal cells derived from adipose tissue, confirmed immunophenotyping panel, were obtained. The average viability rates obtained with propidium iodide was 61.89% while the mortality rate was 32.68% after a cryopreservation cycle.

Conclusions:

Using a cryopreservation protocol with a defined medium with 10% dimethylsulfoxide and animal protein free, it is possible to obtain acceptable viability rates of stromal cells derived from adipose tissue after a cryopreservation cycle.

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