Abstract

PROKOPOWITSCH, I.; CABRALES SALGADO, R.; DIAZ CABALLERO, A.  and  SIMANCAS PALLARES, M.. In vitro histomorphometric comparison of periodontal ligament of extracted premolar teeth preserved in different media storage. Avances en Periodoncia [online]. 2013, vol.25, n.1, pp.41-47. ISSN 2340-3209.

Objective: The aim of this study was to histologically and morphometrically evaluate the results of different types of storage media for avulsed teeth in the maintenance and preserving the integrity of the periodontal ligament. Material and methods: Experimental study. It was used twenty-three extracted premolars for orthodontic reasons. Preserving methods evaluated were: type "B" and "C" milk, saline and dry environment. A total of five teeth were stored in each storage media for 120 minutes. Three teeth served as controls fixed immediately after extraction representing the entire periodontal ligament. The teeth were fixed, processed and stained with hematoxylin/eosin for histological evaluation by light microscopy. Results: The age of the patients who provided the teeth ranged from 13-17 years. The results of the qualitative assessment showed that saline was the most appropriate storage medium, followed by milk type C and type B. In the statistical analysis there was no statistically significant difference between saline and type C milk. After 120 minutes it was found statistically significant differences between histomorphometric changes in the control group and groups of teeth that were kept in wet conditions and dry. Conclusion: Given the results of this study, saline (group II) and milk type C (Group III) can be considered as the most suitable form of storage of avulsed teeth.

Keywords : Periodontics; tooth Injuries; tooth avulsion; alveolar process; periodontal ligament.

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