Abstract

LOZANO BENITO, D.; GIL HUERTA, L.  and  ALVAREZ SAN MARTIN, C.. Effect of seminal plasma addition on frozen-thawed equine semen. Sanid. Mil. [online]. 2011, vol.67, n.3, pp.284-290. ISSN 1887-8571.  https://dx.doi.org/10.4321/S1887-85712011000400005.

Background and objectives: Stallion sperm cryopreservation offers benefits not available in cooled semen. However various factors affect the success of artificial insemination with frozen-thawed equine semen. This study aims to evaluate if adding different concentrations of seminal plasma on frozen-thawed equine spermatozoa affects sperm motility, viability and membrane status. Material and Methods: Different breeds were used; four saddle stallions and two draft stallions. In the first experiment thawed semen was centrifuged and in the second one it was not. Subsequent to that, the spermatozoa resuspended with 10, 20, 30% seminal plasma in phosphate buffered saline and pure seminal plasma (100%). Results: semen parameters of saddle stallions were not affected (p>0,05), but a possible toxic effect of pure seminal plasma was observed on sperm characteristics. Seminal plasma samples in draft breed got better rates in viable sperm with intact acrosome. A lower percentage was also found on spermatozoa with acrosome reaction than in control samples. This category showed signif icant differences (p<0,05). Conclusions: Post-thawing spermatozoa incubation with seminal plasma can stop acrosome reaction, due to the low percentage of spermatozoa suffering true acrosome reaction.

Keywords : Seminal plasma; Frozen-thawed spermatozoa; Equine; Acrosome reaction; Artificial insemination.

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