Abstract

ORTEGA GARCIA, Mª V. et al. Classification in molecular subtypes of breast tumors of small animals through immunohistochemical methods. Sanid. Mil. [online]. 2013, vol.69, n.1, pp.6-12. ISSN 1887-8571.  https://dx.doi.org/10.4321/S1887-85712013000100002.

Antecedents and objectives: to apply an antibodies panel (anti-progesterone receptor -estrogen receptor, -human epidermal growth factor receptor 2 and cytokeratin 14) using immunohistochemical methods in mammary tumors of small animals to classify them in molecular subtypes and their association with the invasion, grade and histological type of the malignancies. Material and Methods: samples of malignant mammary tumors, 10 from canine species and 3 from feline ones. Internal positive control: non-tumoral mammary gland adjacent to the malignancy. Results: 23% (3/13) of the tumors were of the luminal B subtype, 23% (3/13) were HER2 positive, 46% (6/13) were basal types and 7,6% (1/13) were unclassifiable because they did not express any of the tested tumor markers. None of the cases belonged to the luminal A subtype. The 6 basal tumors were grade II or III and presented only stromal infiltration or vascular invasion as well. Two thirds of the HER2 positive tumors presented stromal infiltration and half the tumors were grade II. Two thirds of luminal B tumors were grade II or III. All internal controls were positive. There were no significant differences in the distribution of the molecular subtypes among the different groups of the invasion (p-value=0.26) and malignancy grade variables (p-value=0.42). There were differences of borderline statistical significance in the distribution of the molecular subtypes among the different groups of the histological type variable (p-value=0.08). Conclusions: the application of the antibodies panel has allowed to find 4 (luminal B, HER2, basal and unclassified) out of 5 possible molecular subtypes.

Keywords : Receptor; Molecular subtype; Immunohistochemical method.

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