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Revista de Osteoporosis y Metabolismo Mineral
On-line version ISSN 2173-2345Print version ISSN 1889-836X
Abstract
TORRUBIA, B. et al. Comparison between two automated chemiluminescence immunoassays for quantifying 25(OH) vitamin D. Rev Osteoporos Metab Miner [online]. 2016, vol.8, n.2, pp.70-74. ISSN 2173-2345.
Introduction: Quantifying total blood 25 (OH) vitamin D is the most accurate marker of an individual's vitamin D status. The gold standard technique for measurement is liquid chromatography tandem mass spectrometry (LC-MS/MS), although currently clinical laboratories tend to use chemiluminescence techniques. The objective of this study was to compare 25 (OH) vitamin D concentrations obtained by two commercially-produced automated methods and study the correlation of these methods with the LC-MS/MS reference technique. Material and methods: The 25(OH) vitamin D levels were quantified in 1,000 serum samples from theJimenez Diaz Biochemistry Foundation Laboratory using 2 automated methods for chemiluminescence detection:ADVIA CENTAURO® (SIEMENS) and LUMIPULSE® G1200 (Fujirebio). Among all the samples tested, the 50 most discordant to each other were sent to be evaluated by LC-MS/MS reference technique. Results: The results indicate that there is good correlation between the two methods: CCI=0.923 (0.914-0.932), with the G1200 LUMIPULSE® values 10% being higher than CENTAURO®. Regarding the 50 samples selected, we can see that there is a good correlation between the two immunoassays with LC-MS/MS, although both methods significantly underestimate 25 (OH) vitamin D results with respect to the gold standard. Discussion: Although both techniques are suitable for use, it is worth considering whether the worldwide vitamin D deficiency epidemic is due to the analysis methodology used. This variability between immunoassays could be solved by standardizing the different commercial techniques in line with NIST-produced reference materials.
Keywords : 25(OH) vitamin D; Fujirebio; SIEMENS; technical comparison; LC-MS/MS.
