Abstract

TABLADO-MOLINERA, Álvaro et al. The secretome of mechanically stimulated osteocytes modulates mesenchymal cell function. Rev Osteoporos Metab Miner [online]. 2023, vol.15, n.1, pp.21-28.  Epub May 29, 2023. ISSN 2173-2345.  https://dx.doi.org/10.20960/revosteoporosmetabminer.00007.

The skeleton is a metabolically active organ that is continuously remodeled throughout our lives. This remodeling entails a balance between the formation of bone conducted by the osteoblasts and resorption by osteoclasts. Osteocytes regulate these two processes and their mechanical stimulation is essential to maintain the good functioning of bones and prevent diseases such as osteoporosis. Osteocyte stimulation causes an alteration in the production and secretion of signaling molecules that regulate osteoblast and osteoclast activity. Mesenchymal stem cells have been proposed as a possible cellular therapy for the regeneration of different tissues including bone tissue. We hypothesize in the present study that the secretome of mechanically stimulated osteocytic mouse cells affect the proliferative, adhesive capacity and gene expression of undifferentiated mesenchymal cells and preosteoblastic mesenchymal cells. To that end, the above-mentioned biological processes were analyzed in continuous preosteoblastic cellular lines and mouse mesenchymal cells in the presence of the medium conditioned by MLO-Y4 osteocytic cells after undergoing a mechanical stimulus by fluid flow. It was observed that proliferation increased in both cellular lines in the presence of secretome of mechanically stimulated osteocytes versus control while non-mechanically stimulated osteocytes caused its reduction. It was also possible to observe an increased adhesive capacity of C3H/10T1/2 cells after stimulation with the secretome of mechanically stimulated osteocytes. Regarding gene expression, only the adipogenic factor PPARᵞ underwent alterations in MC3T3-E1 cells by the secretome of osteocytes. These studies indicate that osteocytes can modify the biological behavior of mesenchymal cells by the secretion of soluble factors.

Keywords : Mechanical stimulus; Osteocytes; Mesenchymal cells; Mechanotransduction.

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