Abstract

SANCHEZ NAVARRO, Mª. R.; FERNANDEZ-CONDE, Mª. E.; BLANCO MARTIN, S.  and  SAMANIEGO, C.. Alkaline phosphatase isoenzymes in the serum of patients with renal insufficiency. An. Med. Interna (Madrid) [online]. 2002, vol.19, n.9, pp.11-18. ISSN 0212-7199.

Objective: The aim of this study was to test the utility of serum alkaline phosphatase isoenzymes determination from patients with renal insufficiency.  Material and methods: serum levels of alkaline phosphatase isoenzymes were determined in a group of 58 patients: 22 of them suffering acute renal insufficiency (ARI) and 36 with chronic renal failure (CRF) undergoing regular hemodialysis, results obtained were compared from a population of 30 healthy adults. Intestinal, bone, liver, macromolecular and intestinal variant isoenzymes, were separated by electrophoresis on agarose gel and quantified using a densitometer.  Results: were found a significant increase the total alkaline phosphatase activity in both pathologic groups (p<0.05 in ARI and p<0.01 in CRF). Isoenzymatic profiles showed: increase of the bone fraction (p<0.05 in ARI and p<0.001 in CRF), decrease of the liver isoenzyme (p<0.001) in both groups, macromolecular fraction elevated in acute patients (p<0.05) and a significant increase of the intestinal and intestinal variant isoenzyme in the chronic patients (p<0.001).  Conclusion: the renal insufficiency modified the normal distribution of alkaline phosphatase isoenzymes and the study of their serum levels could be an effective non-invasive marker, for the evaluation of bone disease and intestinal disorders associated with renal failure.

Keywords : Acute renal insufficiency; Chronic renal insufficiency; Alkaline phosphatase isoenzymes; Renal osteodystrophy.

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