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Avances en Odontoestomatología
On-line version ISSN 2340-3152Print version ISSN 0213-1285
Abstract
PAZ PUMPIDO, F. Biocompatibility of the dentin adhesives. Av Odontoestomatol [online]. 2005, vol.21, n.1, pp.339-345. ISSN 2340-3152.
This is a description of the isolation and cultivation of human odontoblasts, and their identification via phenotypic and functional characteristics. Once they have been identified they are frozen in liquid nitrogen. Glass discs are then prepared and three dentin adhesives are applied with a brush, and then a suspension of odontoblasts in a suitable cultivation is poured on and the culture is prepared. The functional characteristics of the cells are studied in terms of DNA synthesis, alkaline phosphate activity, collagen synthesis and matrix formation.
Keywords : Biocompatibility; Odontoblasts; Dentin adhesives.