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Avances en Odontoestomatología
versão On-line ISSN 2340-3152versão impressa ISSN 0213-1285
Resumo
GUTIERREZ NOVA, P.; OLIVARES NAVARRETE, R. e LEYVA HUERTA, E.R.. Epidermal growth factor and total protein in saliva of smokers and non smokers. Av Odontoestomatol [online]. 2008, vol.24, n.6, pp.377-383. ISSN 2340-3152.
The role of Epidermal Growth Factor (EGF) is to maintain tissues integrity. Its receptor is located on cell surfaces and mainly expressed in epithelia. The aim of this study was to determine the association between chronicle tabaquism, pH, total proteins (TP) and EGF in smokers saliva. There were selected male volunteers between 20 and 60 years old from the Registration Clinic of the Postgrade Division of UNAMs Dentistry School. There were taken stimulated (sWHS) and not stimulated whole saliva (WHS) samples from groups of smokers and non smokers. Total proteins (Bradford method), pH and EGF (ELISA Kit KHG0061 biosource) were measured. Results: Slightly alkaline pH in sWHS in both groups. TP were 1.11 ± 0.71 for non smokers WHS and 0.87 ± 0.31 mg/mL for smokers WHS. In non smokers WHS EGF were 14.8 ± 5.62 compared to smokers WHS which showed 11.4 ± 5.45. For sWHS the values were 7.8 ± 5.60 in non smokers group and 9.10 ± 6.53 ng/mL in smokers group.
Palavras-chave : Total proteins; epidermal growth hactor; saliva; smokers.