Meu SciELO
Serviços Personalizados
Journal
Artigo
Indicadores
- Citado por SciELO
- Acessos
Links relacionados
- Citado por Google
- Similares em SciELO
- Similares em Google
Compartilhar
International Microbiology
versão impressa ISSN 1139-6709
Resumo
BERKENHEGER, Imke e FISCHER, Ulrich. Competition for polymers among heterotrophic bacteria, isolated from particles of the Equatorial Atlantic. INT. MICROBIOL. [online]. 2004, vol.7, n.1, pp.13-18. ISSN 1139-6709.
Three heterotrophic bacterial strains, isolated from organic particles of the upper water column of the Equatorial Atlantic, taken during a cruise on the R/V METEOR (1997), were investigated concerning their physiological and phylogenetic properties using classic microbiological and modern molecular-biological methods. All isolates are gram-negative rods able to use polymers such as cellulose, chitin or starch as sole carbon source. The phylogeny of these isolates was investigated by fluorescence in situ hybridization (FISH) and 16S rDNA sequencing. The three isolated strains belong to the Cytophaga/Flavobacteria, γ-Proteobacteria (Marinobacter sp.), and α-Proteobacteria (Sulfitobacter pontiacus). In order to study succession during growth on polymers naturally occurring in marine habitats, FISH was used as a new approach to detect cells from different phylogenetic clusters in the course of a single growth experiment. Mixed cultures consisting of the isolated strains in equal amounts were incubated with cellulose, chitin or starch. Isolate 4301-10/2, a member of the γ-Proteobacteria, dominated in mixed cultures growing on cellulose, chitin, or starch after only 10 days, with 55, 60, and 95%, respectively, of cells hybridizing with 4΄,6-diamidino-2-phenylindole (DAPI).
Palavras-chave : FISH; particle-attached bacteria; marine bacteria; mixed culture; substrate utilization.