Meu SciELO
Serviços Personalizados
Journal
Artigo
Indicadores
- Citado por SciELO
- Acessos
Links relacionados
- Citado por Google
- Similares em SciELO
- Similares em Google
Compartilhar
Journal of Negative and No Positive Results
versão On-line ISSN 2529-850X
Resumo
ECHEVARRIA MARTINEZ, Damelia et al. On the discriminative capacity of several autoantibodies for the diagnosis of Systemic Lupus Erythematosus. JONNPR [online]. 2020, vol.5, n.11, pp.1333-1355. Epub 18-Dez-2023. ISSN 2529-850X. https://dx.doi.org/10.19230/jonnpr.3657.
Rationale.
Several autoantibodies have been proposed for the differential diagnosis of Systemic Lupus Erythematosus (SLE). Use of these autoantibodies might result in different operational characteristics.
Objective.
To assess the operational characteristics of selected autoantibodies in the differential diagnosis of SLE.
Study design.
Retrospective, analytical.
Study serie.
One-hundred and sixty-nine subjects (SLE: 35.5 %; Non-SLE diseases of connective tissue: 38.5 %; Apparently healthy subjects: 26.0 %; Women: 84.6 %; Average age: 30.9 ± 17.9 years; Average evolution time of lupic disease: 3.9 ± 4.7 years).
Methods.
Anti-double stranded DNA (anti-dsDNAdc), anti-nucleosomes (anti-Nuc), anti-Smith protein (anti-Sm) and anti-ribosomes (anti-Rib) autoantibodies were determined by immunoenzymatic techniques (Orgentec Diagnostika, Mainz, Alemania). Anti-nuclear antibodies (ANA) were indistinctely determined either by immunoenzimatic techniques (ANA-EIA) or immunofluorescent methods with Hep2 cells (ANAHep2). Operational characteristics of the autoantibodies in the differential diagnosis of SLE were calculated. In addition, corresponding ROC curves for each instance of analysis were obtained, and diagnostic accuracy was estimated from the area under the ROC curve (AROC).
Results.
Accuracy of the autoantibodies used in the differential diagnosis of SLE was as follows (in descending order): anti-dsDNA: 0.9528 ± 0.0288; anti-Nuc: 0.7851 ± 0.0675; ANA-EIA: 0.7755 ± 0.2633; anti-Sm: 0.5926 ± 0.0790; anti-Rib: 0.5770 ± 0.2641; and ANA-Hep2: 0.5341 ± 0.2635; respectively. With the exception of anti-dcDNA, operational characteristics of the remaining antibodies were dependent upon cut-off value, sampled subpopulation, and analytical method.
Conclusions.
anti-dsDNA antibodies were the most accurate in the differential diagnosis of SLE.
Palavras-chave : Systemic Lupus Erythematosus; Autoantibodies; Operational characteristics; ROC curve.