Abstract

MARQUEZ ARAQUE, A.T. et al. Degradation of fibrolytic enzymes from Trametes sp. EUM1, Pleurotus ostreatus IE8 and Fibrozyme^®. Arch. zootec. [online]. 2010, vol.59, n.225, pp.145-148. ISSN 1885-4494.

Ruminal degradation of enzymes produced by fungi Trametes sp. EUM1, Pleurotus ostreatus IE8, and from a commercial product (Fibrozyme^®) was determined trough ammonia nitrogen (N-NH₃) released. For each enzyme results were analyzed as a factorial experiment (3x2x2); factors were: substrate (xylan, carboximethycellulose and no substrate), protein level of enzyme (1 or 2) and times of contact enzyme-substrate previous incubation (0 or 1 h). The NH₃-N concentration in ruminal fluid was different (p<0.05) between substrates on all studied enzymes. The protein level of enzyme had effect (p<0.05) on NH₃-N concentration from Trametes sp. or P. ostreatus enzymes, but not from Fibrozyme. The substrate by protein level had effect (p<0.05) in the NH₃-N concentration in the Trametes sp. and P. ostreatus enzymes. The time of contact enzyme-substrate effect was not significant for any of studied enzymes. Because minor N-NH₃ concentration was observed when enzymes were incubated with a substrate, this indicates that degradation of enzymes in ruminal liquid was reduced in presence of a substrate; therefore substrate is important in the stability of studied enzymes.

Keywords : Fungi; Substrate; Ammoniacal N.

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