Abstract

MATOS-BRITO, B.G. et al. Effect of initial seminal plasma fructose concentration on goat semen storage at 5^oC. Arch. zootec. [online]. 2013, vol.62, n.237, pp.143-146. ISSN 1885-4494.  https://dx.doi.org/10.4321/S0004-05922013000100015.

Twenty-four goat semen samples were collected and divided into four aliquots, diluted with the citrate-egg yolk (CY), TRIS-egg yolk (TY) or industrialized coconut water with egg yolk (ICW-Y) extenders. The fourth aliquot was centrifuged to analyze fructose concentration and PLA₂ activity on SP. The semen was stored at 5^oC and evaluated at times fresh, 2, 24 and 48 h, in each time was evaluated the vigor, sperm motility and total morphological alterations. The animals were divided into two groups: group Ifructose concentration >710 mg/dL and group IIfructose concentration <480 mg/dL. The ICW-Y extender presented inferior results. The effect of the group was observed only in the ICW-Y. CY and TY worked similarly in the two groups. No significant difference in the activity of PLA₂ was found between the groups. However, ICW-Y should not be used for the storage of goat semen.

Keywords : Citrate-egg yolk; Industrialized coconut wateregg yolk; Phospholipase A₂; TRIS-egg yolk.

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