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Archivos de Zootecnia
versión On-line ISSN 1885-4494versión impresa ISSN 0004-0592
Resumen
MATOS-BRITO, B.G. et al. Effect of initial seminal plasma fructose concentration on goat semen storage at 5oC. Arch. zootec. [online]. 2013, vol.62, n.237, pp.143-146. ISSN 1885-4494. https://dx.doi.org/10.4321/S0004-05922013000100015.
Twenty-four goat semen samples were collected and divided into four aliquots, diluted with the citrate-egg yolk (CY), TRIS-egg yolk (TY) or industrialized coconut water with egg yolk (ICW-Y) extenders. The fourth aliquot was centrifuged to analyze fructose concentration and PLA2 activity on SP. The semen was stored at 5oC and evaluated at times fresh, 2, 24 and 48 h, in each time was evaluated the vigor, sperm motility and total morphological alterations. The animals were divided into two groups: group Ifructose concentration >710 mg/dL and group IIfructose concentration <480 mg/dL. The ICW-Y extender presented inferior results. The effect of the group was observed only in the ICW-Y. CY and TY worked similarly in the two groups. No significant difference in the activity of PLA2 was found between the groups. However, ICW-Y should not be used for the storage of goat semen.
Palabras clave : Citrate-egg yolk; Industrialized coconut wateregg yolk; Phospholipase A2; TRIS-egg yolk.