Abstract

MATHISON NATERA, Y. et al. Pentosan polysulfate sodium prevents kidney morphological changes and albuminuria in rats with type 1 diabetes. Nefrología (Madr.) [online]. 2010, vol.30, n.6, pp.639-645. ISSN 1989-2284.

Decreased levels of glycosaminoglycans (GAG) have been observed in kidney and other organs, in human and animal models of diabetes. Long term administration of heparins and other glycosaminoglycans have demonstrated a beneficial effect on morphological and functional renal abnormalities in diabetic rats. We assessed the effect of sodium pentosan polysulfate (SPP), a semi-synthetic glycosaminoglycan with low anticoagulant activity, on renal involvement in streptozotocin diabetic rats. Diabetes was induced in male Sprague-Dawley rats by i.v. administration of streptozotocin (STZ). Animals were randomly allocated in three groups: C = control, STZ and STZ + SPP = pretreated with SPP (15 mg/kg, s.c.). After three months of follow-up, blood and 24 h-urine samples were obtained and then the animals were sacrificed and the kidney microdissected for morphometric analysis. Urinary albumin excretion was markedly increased in untreated diabetic rats (C = 0.26 ± 0.03 vs. STZ = 7.75 ± 1.8 mg/24 h) and SPP treatment partially prevented the albumin rise (3.7 ± 0.7 mg/24 h), without affecting the metabolic control HbA_1c (C = 3.6 ± 1.7; STZ = 8.82 ± 0.47; STZ + SPP = 8.63 ± 0.54). Electron microscope observation revealed typical renal lesions described in experimental diabetes (STZ group). SPP administration prevent the tubular basement membrane thickening and the lost of cytoarchitecture induced by experimental diabetes. Our data demonstrated that long-term administration of SPP have a favorable effect on morphological and functional abnormalities in kidney of diabetic rats and suggests a potential therapeutic use for this compound.

Keywords : Microalbuminuria; Glycosaminoglycans; kidney damage; Diabetes.

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