Antioxidant responses of damiana (Turnera diffusa Willd) to exposure to artificial ultraviolet (UV) radiation in an in vitro model: part I; UV-C radiation

Soriano-Melgar, Lluvia de Abril Alexandra; Alcaraz-Meléndez, Lilia; Méndez-Rodríguez, Lía C.; Puente, María Esther; Rivera-Cabrera, Fernando; Zenteno-Savín, Tania

doi:10.3305/nh.2014.29.5.7093

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Nutrición Hospitalaria

On-line version ISSN 1699-5198Print version ISSN 0212-1611

Abstract

SORIANO-MELGAR, Lluvia de Abril Alexandra et al. Antioxidant responses of damiana (Turnera diffusa Willd) to exposure to artificial ultraviolet (UV) radiation in an in vitro model: part I; UV-C radiation. Nutr. Hosp. [online]. 2014, vol.29, n.5, pp.1109-1115. ISSN 1699-5198. https://dx.doi.org/10.3305/nh.2014.29.5.7093.

Introduction: Ultraviolet type C (UV-C) radiation has higher energy than the UV-B radiation and has been less studied because it is completely absorbed by the ozone layer. However, artificial UV-C radiation can generate diverse modifications in the plants. Given that exposure to UV-C for short periods of time increases the antioxidant content, improving the appearance and shelf-life of products, its potential application in postharvest treatments to modify the antioxidant content of medicinal plants, such as damiana (Turnera diffusa), is novel and relevant. Objective: To determine the effects of UV-C radiation on enzymatic and non-enzymatic antioxidant defenses, as well as oxidative damage levels, in damiana (Turnera diffusa) plants in vitro. Results: UV-C radiation decreased superoxide dismutase (SOD, EC 1.15.1.1) and total peroxidases (POX, EC 1.11.1) activities, the concentration of chlorophylls (a and b), carotenes, vitamin C, and total antioxidant capacity. UV-C radiation increased the phenolic compound levels in damiana. Loss of antioxidant defenses was higher in damiana plants exposed to higher UV-C doses and/or for longer periods. This study suggests that UV-C radiation induces oxidative stress, evidenced as increased protein carbonyls and phenolic compound content, in damiana (T. diffusa). Conclusion: Low dose, short exposure to UV-C stimulates phenolic compound content in damiana. Thus, controlled UV-C treatments could be used as postharvest treatment to increase phenolic compound content in damiana plants.

Keywords : Antioxidants; Antioxidant capacity; Phenolic compounds; Turnera diffusa; UV-C radiation; Vitamin C.

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