Abstract

SANCHEZ NAVARRO, M. R.; FERNANDEZ-CONDE, E.  and  SAMANIEGO, C.. Isoamylases and isolipases valoration in the serum from patients with renal insufficiency. An. Med. Interna (Madrid) [online]. 2001, vol.18, n.9, pp.13-19. ISSN 0212-7199.

Ojective: The aim of this study is to test the utility os serum isoamylases and isolipases as determinated from patients with renal insufficiency. Material and methods: Serum levels of isoamylases and isolipases were determined in a group of sixty-eight patiens with renal disease, 32 of them suffering acute insufficiency and 36 with chronic renal failure undergoing regular hemodialysis, results obtained were compared from a population of 44 healthy adults. We used a new method for isolipases determination in serum based on its separation on agarose gel. Two forms of lipase, L1 and L2, were identified by this method and queantitated by densitometry. Results: Were found a significant increase of pancreatic isoamylase P2 and P/S isoenzymatic ratio in acute patients (p<0.001) as chronic (p<0.005). In both groups, the isolipase L1 activity and L1/L2 isoform ratio were showed significantly elevated (p<0.01). We studied the relationship between isoamylases and isolipases establishing the P2/L2 ratio (normal range <0.6) showing, in the two pathologic groups, significantly elevated values compared with the control group (p<0.001) and a positive and sifnificant correlation between the P2/L2 and P/S isoform ratios (r=0.76, p<0.005 in acute patiens; r=0.58, p<0.05 in chrónic patiens).  Conclusion: The combined study of serum levels of isoamylases and isolipases could be an effective marker for diagnosis and evolution of associated pancreatitis with acute or chronic renal failure.

Keywords : Isoamylases; Isolipases; Renal insufficiency.

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